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Literature summary for 1.1.5.4 extracted from

  • Hartuti, E.D.; Inaoka, D.K.; Komatsuya, K.; Miyazaki, Y.; Miller, R.J.; Xinying, W.; Sadikin, M.; Prabandari, E.E.; Waluyo, D.; Kuroda, M.; Amalia, E.; Matsuo, Y.; Nugroho, N.B.; Saimoto, H.; Pramisandi, A.; Watanabe, Y.I.; Mori, M.; Shiomi, K.; Balogun, E.O.; Shiba, T.; Harada, S.; Nozaki, T.; Kita, K.
    Biochemical studies of membrane bound Plasmodium falciparum mitochondrial L-malate quinone oxidoreductase, a potential drug target (2018), Biochim. Biophys. Acta Bioenerg., 1859, 191-200 .
    View publication on PubMed
Search for more literature for 1.1.5.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in an Escherichia coli BL21Star(DE3) Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
ferulenol inhibits parasite growth and shows strong synergism in combination with atovaquone, an anti-malarial and bc1 complex inhibitor Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 decylubiquinone pH 7.0, 37°C Plasmodium falciparum
5.99
-
 (S)-malate pH 7.0, 37°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane bound Plasmodium falciparum 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + ubiquinone Plasmodium falciparum the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) oxaloacetate + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6KT09 isolate 3D7
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + decylubiquinone
-
 Plasmodium falciparum oxaloacetate + decylubiquinol
-
 ?
(S)-malate + ubiquinone the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) Plasmodium falciparum oxaloacetate + ubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
L-malate:quinone oxidoreductase
-
 Plasmodium falciparum
PfMQO
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Plasmodium falciparum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 25-30°C: about 50% of maximal activity, 40°C: about 90% of maximal activity Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.2 no significant difference in activity from pH 6.8 to 8.2 Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00925
-
 pH 7.0, 37°C Plasmodium falciparum ferulenol

General Information

General Information Comment Organism
drug target the enzyme is a potential antimalarial drug target against the apicomplexan parasite Plasmodium falciparum, because the enzyme is absent in humans. Ferulenol inhibits parasite growth and shows strong synergism in combination with atovaquone, an anti-malarial and bc1 complex inhibitor Plasmodium falciparum
metabolism the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) Plasmodium falciparum
physiological function the enzyme is essential for parasite survival, at least, in the intra-erythrocytic asexual stage Plasmodium falciparum