Crystallization (Comment) | Organism |
---|---|
structures in complexes with isoeugenol, coniferyl alcohol, vanillin, and benzoate. The catalytic center is a remarkable solvent-inaccessible cavity on the si side of the flavin cofactor. Structural comparison with vanillyl alcohol oxidase from Penicillium simplicissimum | Rhodococcus jostii |
Organic Solvent | Comment | Organism |
---|---|---|
diisopropyl ether | increases selectivtiy and conversion rate of the reaction with 4-(1-hydroxyethyl)-2-methoxyphenol | Rhodococcus jostii |
DMSO | 10% (v/v), increase in the enzymatic activity | Rhodococcus jostii |
Ethyl acetate | increase in enzymatic activity | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SBK1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(1-hydroxyethyl)-2-methoxyphenol + O2 | racemic analogue of vanillyl alcohol | Rhodococcus jostii | 1-(4-hydroxy-3-methoxyphenyl)ethan-1-one + 4-[(1S)-1-hydroxyethyl]-2-methoxyphenol + ? | - |
? | |
4-(4-hydroxyphenyl)butan-2-one + O2 | - |
Rhodococcus jostii | (3E)-4-(4-hydroxyphenyl)but-3-en-2-one + ? | - |
? | |
eugenol + O2 + H2O | - |
Rhodococcus jostii | coniferyl alcohol + H2O2 | - |
? | |
indan-5-ol + O2 | - |
Rhodococcus jostii | 1H-inden-6-ol + 5-hydroxyindan-1-one + ? | - |
? | |
additional information | EUGO can efficiently catalyze the dehydrogenation of various phenolic ketones and the selective oxidation of a racemic 4-(1-hydroxyethyl)-2-methoxyphenol. Very poor substrates: 4-(1,3-dithiolan-2-yl)phenol, capsaicin, (4-hydroxyphenyl)acetonitrile, 3-(4-hydroxyphenyl)propanoic acid, 2-methoxy-4-methylphenol, and 4-(hept-1-enyl)-2-methoxyphenol | Rhodococcus jostii | ? | - |
- |
|
vanillyl alcohol + O2 | - |
Rhodococcus jostii | vanillin + H2O2 | - |
? | |
zingerone + O2 | - |
Rhodococcus jostii | dehydrozingerone + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EUGO | - |
Rhodococcus jostii |
RHA1_ro03282 | - |
Rhodococcus jostii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
melting temperature, pH 5-8. The presence of 10% (v/v) DMSO or ethyl acetate does not significantly affect the enzyme's thermostability | Rhodococcus jostii |