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Literature summary for 1.1.3.7 extracted from
- Expression of Pleurotus eryngii aryl-alcohol oxidase in Aspergillus nidulans purification and characterization of the recombinant enzyme (2001), Biochim. Biophys. Acta, 1546, 107-113 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Aspergillus nidulans | Pleurotus eryngii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| synthesis | expression of AAO in the ascomycete Aspergillus nidulans. The activity of the recombinant enzyme in Aspergillus nidulans cultures is much higher than found in the extracellular fluid of Pleurotus eryngii. The recombinant enzyme shows the same molecular mass, pI and catalytic properties as that of the mature protein secreted by Pleurotus eryngii | Pleurotus eryngii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
4-anisyl alcohol | recombinant enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii |  |
| 0.04 | - |
4-anisyl alcohol | native enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.22 | - |
3-anisyl alcohol | native enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.3 | - |
3-anisyl alcohol | recombinant enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.41 | - |
veratryl alcohol | native enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.56 | - |
veratryl alcohol | recombinant enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.63 | - |
benzyl alcohol | recombinant enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
| 0.85 | - |
benzyl alcohol | native enzyme, pH 6, temperature not specified in the publication | Pleurotus eryngii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Pleurotus eryngii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus eryngii | O94219 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains about 14% N-linked carbohydrates | Pleurotus eryngii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-anisyl alcohol + O2 | - |
Pleurotus eryngii | 3-anisyl aldehyde + H2O2 | - |
? | |
| 4-anisyl alcohol + O2 | - |
Pleurotus eryngii | 4-anisyl aldehyde + H2O2 | - |
? | |
| benzyl alcohol + O2 | - |
Pleurotus eryngii | benzyl aldehyde + H2O2 | - |
? | |
| veratryl alcohol + O2 | - |
Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72500, SDS-PAGE | Pleurotus eryngii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pleurotus eryngii | isoelectric focusing | - |
3.8 |
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