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Literature summary for 1.1.3.4 extracted from

  • Yuan, M.; Ning, C.; Yang, S.; Liang, Q.; Mou, H.; Liu, Z.
    A new cold-active glucose oxidase from Penicillium high-level expression and application in fish preservation (2020), Front. Microbiol., 11, 606007 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.4

Application

Application Comment Organism
food industry potentially useful in food biopreservation, application for the preservation of aquatic products at low-temperatures, good antimicrobial effect against common fish pathogenic bacteria (Listeria monocytogenes and Vibrio parahaemolyticus), excellent freshness preserving agent in the context of the grass carp Penicillium sp. MX3343

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris X33 Penicillium sp. MX3343

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
65.7
-
 beta-D-glucose pH 6.0, 30°C Penicillium sp. MX3343

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 Penicillium sp. MX3343
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp. MX3343 A0A7L7T1A0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp. MX3343

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
149.05
-
 pH 6.0, 30°C, substrate: beta-D-glucose Penicillium sp. MX3343

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2
-
 Penicillium sp. MX3343 D-glucono-1,5-lactone + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
GOxP5
-
 Penicillium sp. MX3343

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Penicillium sp. MX3343

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 30 4°C: about 72% of maximal activity, 30°C: maximal activity, recombinant enzyme Penicillium sp. MX3343

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
137.1
-
 beta-D-glucose pH 6.0, 30°C Penicillium sp. MX3343

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 recombinant enzyme Penicillium sp. MX3343

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 6 stable, recombinant enzyme Penicillium sp. MX3343

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.087
-
 beta-D-glucose pH 6.0, 30°C Penicillium sp. MX3343