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Literature summary for 1.1.3.4 extracted from

  • Mano, N.
    Engineering glucose oxidase for bioelectrochemical applications (2019), Bioelectrochemistry, 128, 218-240 .
    View publication on PubMed
Search for more literature for 1.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed either in Escherichia coli or in Pichia pastoris Penicillium amagasakiense
overexpression in Saccharomyces cerevisiae Aspergillus niger

Protein Variants

Protein Variants Comment Organism
A173T/A332S increased electron transfer (1.2fold) Aspergillus niger
A173T/F414L increased electron transfer (1.2fold), 70% decrease in O2 sensitivity Aspergillus niger
A173V/A332S/F414I/V560T increased electron transfer (6.4fold), decrease in O2 sensitivity Aspergillus niger
A332S/V560T increased electron transfer (1.2fold), 70% decrease in O2 sensitivity Aspergillus niger
F414Y increased electron transfer Aspergillus niger
I94V/T30S increased O2 sensitivity, increased electron transfer (1.9fold) Aspergillus niger
K424E increased electron transfer (2.4fold), 20% decrease in O2 sensitivity Penicillium amagasakiense
S114A/F355L increased electron transfer, 88% decrease in O2 sensitivity Penicillium amagasakiense
T110A the mutant enzyme displays 12.3fold reduced O2 consumption Aspergillus niger
T110S increased electron transfer Aspergillus niger
T110S/T34V increased electron transfer Aspergillus niger
T110S/V20Y increased O2 sensitivity Aspergillus niger
T56V/T132S mutant enzyme displays better catalytic properties than the native enzyme Aspergillus niger
V20Y increased electron transfer Aspergillus niger
V464A/K424E 2.4fold increase in electron transfer, 95% decrease in O2 sensitivity Penicillium amagasakiense
V564S 1.1fold increase in electron transfer, 88% decrease in O2 sensitivity Penicillium amagasakiense

Organism

Organism UniProt Comment Textmining
Aspergillus niger P13006
-
-
Penicillium amagasakiense P81156
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Penicillium amagasakiense
glycoprotein eight potential N-glycosylation at N43, N89, N161, N168, N258, N355, N388, and N473 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
GOX
-
 Penicillium amagasakiense

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Aspergillus niger
FAD
-
 Penicillium amagasakiense