Any feedback?
Literature summary for 1.1.3.38 extracted from
- Screening and evaluation of new hydroxymethylfurfural oxidases for furandicarboxylic acid production (2020), Appl. Environ. Microbiol., 86, e00842 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methylovorus sp. MP688 |
| expression in Escherichia coli | Pseudomonas sp. 11/12A |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas sp. 11/12A |  |
| 0.5 | - |
Vanillyl alcohol | pH 7, 25°C | Methylovorus sp. MP688 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylovorus sp. MP688 | E4QP00 | cf. EC 1.1.3.47 | - |
| Pseudomonas sp. 11/12A | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
recombinant protein, substrate vanillyl alcohol, pH 7.0, 25°C | Pseudomonas sp. 11/12A |
| 3.3 | - |
recombinant protein, substrate vanillyl alcohol, pH 7.0, 25°C | Methylovorus sp. MP688 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vanillyl alcohol + O2 | - |
Methylovorus sp. MP688 | vanillin + H2O2 | - |
? | |
| vanillyl alcohol + O2 | - |
Pseudomonas sp. 11/12A | vanillin + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, MALDI-TOF | Methylovorus sp. MP688 |
| ? | x * 57000, MALDI-TOF | Pseudomonas sp. 11/12A |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMFO | - |
Methylovorus sp. MP688 |
| HMFO | - |
Pseudomonas sp. 11/12A |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Pseudomonas sp. 11/12A |
| 45 | - |
- |
Methylovorus sp. MP688 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | more than 80% of maximum activity | Methylovorus sp. MP688 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
melting temperature | Pseudomonas sp. 11/12A |
| 48 | - |
melting temperature | Methylovorus sp. MP688 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 19.5 | - |
Vanillyl alcohol | pH 7, 25°C | Methylovorus sp. MP688 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Methylovorus sp. MP688 |
| 7.5 | 8 | - |
Pseudomonas sp. 11/12A |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
complete loss of activity | Pseudomonas sp. 11/12A |
| 10 | - |
5% residual activity | Methylovorus sp. MP688 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Methylovorus sp. MP688 | |
| FAD | - |
Pseudomonas sp. 11/12A | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methylovorus sp. MP688 | calculated from sequence | - |
6.1 |
| Pseudomonas sp. 11/12A | calculated from sequence | - |
6.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.3 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 36.2 | - |
Vanillyl alcohol | pH 7, 25°C | Methylovorus sp. MP688 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
