BRENDA - Enzyme Database
show all sequences of 1.1.3.21

Purification and properties of L-alpha-glycerophosphate oxidase from Streptococcus faecium ATCC 12755

Esders, T.W.; Michrina, C.A.; J. Biol. Chem. 254, 2710-2715 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
increasing the buffer concentration from 10 mM to 100 mM leads to an 2.6-10fold increase in activity, this effect is observed with potassium phosphate, sodium acetate, sodium maleate or other anionic buffers
Enterococcus faecium
Inhibitors
Inhibitors
Commentary
Organism
Structure
fructose-1-phosphate
-
Enterococcus faecium
fructose-6-phosphate
-
Enterococcus faecium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
sn-glycerol-3-phosphate
-
Enterococcus faecium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CaCl2
10 mM, 7.7fold activation
Enterococcus faecium
CoCl2
10 mM, 10.2fold activation
Enterococcus faecium
KCl
100 mM, 5.8fold activation
Enterococcus faecium
MgCl2
10 mM, 6.8fold activation
Enterococcus faecium
MnCl2
10 mM, 7.6 fold activation
Enterococcus faecium
NaCl
100 mM, 3.8fold activation
Enterococcus faecium
ZnCl2
1 mM, 6.3fold activation
Enterococcus faecium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
2 * 72000, SDS-PAGE
Enterococcus faecium
131000
-
strain ATCC 12755, gel filtration, sucrose density centrifugation
Enterococcus faecium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sn-glycerol-3-phosphate + O2
Enterococcus faecium
-
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
Enterococcus faecium
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterococcus faecium
-
ATCC 12755
-
Purification (Commentary)
Commentary
Organism
-
Enterococcus faecium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
95
strain ATCC 12755
Enterococcus faecium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol-3-phosphate + O2
-
287564
Enterococcus faecium
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
287564
Enterococcus faecium
?
sn-glycerol-3-phosphate + O2
high specificity for L-alpha-glycerophosphate
287564
Enterococcus faecium
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
287564
Enterococcus faecium
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 72000, SDS-PAGE
Enterococcus faecium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Enterococcus faecium
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
2 mol bound per mol of enzyme; prosthetic group
Enterococcus faecium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
increasing the buffer concentration from 10 mM to 100 mM leads to an 2.6-10fold increase in activity, this effect is observed with potassium phosphate, sodium acetate, sodium maleate or other anionic buffers
Enterococcus faecium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
2 mol bound per mol of enzyme; prosthetic group
Enterococcus faecium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fructose-1-phosphate
-
Enterococcus faecium
fructose-6-phosphate
-
Enterococcus faecium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
sn-glycerol-3-phosphate
-
Enterococcus faecium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CaCl2
10 mM, 7.7fold activation
Enterococcus faecium
CoCl2
10 mM, 10.2fold activation
Enterococcus faecium
KCl
100 mM, 5.8fold activation
Enterococcus faecium
MgCl2
10 mM, 6.8fold activation
Enterococcus faecium
MnCl2
10 mM, 7.6 fold activation
Enterococcus faecium
NaCl
100 mM, 3.8fold activation
Enterococcus faecium
ZnCl2
1 mM, 6.3fold activation
Enterococcus faecium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
2 * 72000, SDS-PAGE
Enterococcus faecium
131000
-
strain ATCC 12755, gel filtration, sucrose density centrifugation
Enterococcus faecium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sn-glycerol-3-phosphate + O2
Enterococcus faecium
-
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
Enterococcus faecium
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Enterococcus faecium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
95
strain ATCC 12755
Enterococcus faecium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol-3-phosphate + O2
-
287564
Enterococcus faecium
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
287564
Enterococcus faecium
?
sn-glycerol-3-phosphate + O2
high specificity for L-alpha-glycerophosphate
287564
Enterococcus faecium
glycerone phosphate + H2O2
i.e. dihydroxyacetone phosphate
287564
Enterococcus faecium
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 72000, SDS-PAGE
Enterococcus faecium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Enterococcus faecium
Other publictions for EC 1.1.3.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Pundir
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-
-
-
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1
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5
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1
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1
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3
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1
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-
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740385
Narwal
An improved amperometric trigl ...
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Enzyme Microb. Technol.
100
11-16
2017
-
1
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1
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1
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1
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1
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1
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1
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740092
Samartsev
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Features of inhibition of glyc ...
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9
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-
-
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-
3
-
1
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1
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1
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1
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3
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1
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1
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1
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1
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-
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740154
Li
Characterization of glycerol p ...
Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
25
504-507
2015
-
-
1
-
-
-
1
-
-
-
1
1
-
2
-
-
1
-
-
-
-
-
1
1
1
1
-
-
1
-
-
1
-
-
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1
1
-
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1
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1
1
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1
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-
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
741462
Mulongo
Cattle immunized against the p ...
Mycoplasma mycoides, Mycoplasma mycoides B237
Vaccine
31
5020-5025
2013
-
-
1
-
-
-
-
-
1
-
1
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
1
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-
-
-
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1
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1
2
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1
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712308
Bizzini
Glycerol is metabolized in a c ...
Enterococcus faecalis
J. Bacteriol.
192
779-785
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698631
Hames
Glycerol metabolism is importa ...
Mycoplasma pneumoniae, Mycoplasma pneumoniae M129
J. Bacteriol.
191
747-753
2009
1
-
1
-
1
-
1
-
3
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
1
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1
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1
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1
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1
1
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1
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1
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3
-
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2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
711805
Riboulet-Bisson
Ers controls glycerol metaboli ...
Enterococcus faecalis
Curr. Microbiol.
58
201-204
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
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1
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1
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1
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1
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-
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-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713579
Bischof
Functional and antigenic prope ...
Mycoplasma mycoides subsp. mycoides, Mycoplasma mycoides subsp. mycoides SC Afade
Vet. Res.
40
35
2009
-
-
1
-
1
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-
-
-
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2
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5
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1
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2
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1
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1
1
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1
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2
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
685288
Colussi
Structure of alpha-glycerophos ...
Streptococcus sp.
Biochemistry
47
965-977
2008
-
-
1
1
1
-
1
6
2
-
-
1
-
5
-
-
1
-
-
-
1
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
1
-
6
2
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
688443
Aragon Casale
-
Characterization of glycerol k ...
Saccharomyces cerevisiae
J. Mol. Catal. B
52-53
113-120
2008
3
-
-
-
-
-
-
-
-
-
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1
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1
1
1
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1
1
1
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1
1
1
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3
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1
1
1
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1
1
1
-
1
1
1
-
-
-
-
-
-
-
698226
Minaksh
Co-immobilization of lipase, g ...
Aerococcus viridans
Indian J. Biochem. Biophys.
45
111-115
2008
-
1
-
-
1
-
-
1
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1
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1
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1
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1
2
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1
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1
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1
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1
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1
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1
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1
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1
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1
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
671316
Llaudet
Microelectrode biosensor for r ...
Aerococcus viridans
Anal. Chem.
77
3267-3273
2005
-
-
-
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674249
Pilo
A metabolic enzyme as a primar ...
Mycoplasma mycoides subsp. mycoides
J. Bacteriol.
187
6824-6831
2005
-
-
-
-
-
-
-
-
-
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1
-
-
7
-
-
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
1
-
-
-
-
-
-
655693
Lee
-
Production of L-alpha-glycerop ...
Enterococcus faecium, Enterococcus faecium M74-LC
Han'guk Sikp'um Yongyang Kwahak Hoechi
32
684-688
2003
-
-
-
-
-
-
-
-
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2
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1
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654056
Finnerty
Crystallization and preliminar ...
Streptococcus sp.
Acta Crystallogr. Sect. D
58
165-166
2002
-
-
-
1
-
-
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3
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1
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1
1
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-
-
-
-
-
-
-
-
655707
Kalia
Co-immobilization of lipase, g ...
Aerococcus viridans
Indian J. Biochem. Biophys.
39
342-346
2002
-
-
-
-
-
1
-
-
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-
-
-
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5
-
-
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-
4
-
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1
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1
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4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287570
Streitenberger
Highly efficient Aerococcus vi ...
Aerococcus viridans, Aerococcus viridans CETC 978
Appl. Microbiol. Biotechnol.
57
329-333
2001
-
-
-
-
-
-
4
1
-
-
1
2
-
3
-
-
1
-
-
-
1
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
1
2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287569
Mackova
Properties and stability of gl ...
Aerococcus viridans, Aerococcus viridans DBM 1509
Lett. Appl. Microbiol.
30
188-191
2000
2
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
2
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1
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1
-
1
1
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-
-
2
-
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1
-
-
-
-
-
-
-
-
-
-
2
-
-
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1
2
-
1
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1
-
1
-
-
-
-
-
-
-
287568
Krämer
-
Coimmobilization of L-alpha-gl ...
Pediococcus sp.
Tetrahedron
53
14645-14650
1997
-
1
-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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287567
Wadher
A mutant of Mycoplasma mycoide ...
Mycoplasma mycoides, Mycoplasma mycoides T1, no activity in Mycoplasma mycoides, no activity in Mycoplasma mycoides G1
FEMS Microbiol. Lett.
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127-130
1990
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5
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287565
Claiborne
Studies on the structure and m ...
Enterococcus faecium
J. Biol. Chem.
261
14398-14407
1986
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3
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1
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3
1
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287564
Esders
Purification and properties of ...
Enterococcus faecium
J. Biol. Chem.
254
2710-2715
1979
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1
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7
2
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7
2
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287563
Koditschek
alpha-Glycerophosphate oxidase ...
Enterococcus faecium, Enterococcus faecium F24
J. Bacteriol.
98
1063-1068
1969
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4
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287566
Jacobs
The purification and propertie ...
Enterococcus faecalis 10C1, Enterococcus faecalis
Arch. Biochem. Biophys.
88
250-255
1960
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4
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8
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