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Literature summary for 1.1.3.2 extracted from

  • Streitenberger, S.A.; Lopez-Mas, J.A.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    Non-linear slow-binding inhibition of Aerococcus viridans lactate oxidase by Cibacron Blue 3GA (2001), J. Enzyme Inhib., 16, 301-312 .
    View publication on PubMed
Search for more literature for 1.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
(R)-lactate
-
 Aerococcus viridans
alpha-hydroxybutanoate
-
 Aerococcus viridans
Cibacron blue 3GA at low concentrations, Cibacron Blue 3GA behaves as a dye-, pH- and time-dependent inhibitor, non-linear inhibition Aerococcus viridans
glycolate
-
 Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.157
-
 (S)-lactate pH 7.0, 37°C Aerococcus viridans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
187000
-
 gel filtration Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans D4YFM2
-
-
Aerococcus viridans ATCC 11563 D4YFM2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aerococcus viridans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
114.5
-
 pH 7.0, 37°C Aerococcus viridans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?
(S)-lactate + O2
-
 Aerococcus viridans ATCC 11563 pyruvate + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Aerococcus viridans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Aerococcus viridans

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Aerococcus viridans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.32
-
 glycolate pH 7.0, 37°C Aerococcus viridans
9.04
-
 (R)-lactate pH 7.0, 37°C Aerococcus viridans
42.4
-
 alpha-hydroxybutanoate pH 7.0, 37°C Aerococcus viridans