Application | Comment | Organism |
---|---|---|
drug development | in humans the enzyme is a potential drug target for treatment of primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H260A | site-directed mutagenesis | Homo sapiens |
H260Q | site-directed mutagenesis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloride | inhibits the enzyme at high concentrations | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow, steady-state, and presteady-state kinetics at different pH values, pH profiles, detailed overview | Homo sapiens | |
0.2 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
0.23 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
0.44 | - |
O2 | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
0.59 | - |
O2 | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
0.64 | - |
O2 | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | Homo sapiens | - |
glyoxylate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UJM8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by nickel affinity chromatography, ammonium sulfate fractionation and dialysis | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 | the pH affects the kinetic steps of the catalytic mechanism of human glycolate oxidase, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview. Formation of the enzyme-substrate complex suggests the presence of a protonated group participating in substrate binding | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | - |
Homo sapiens | glyoxylate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycolate oxidase | - |
Homo sapiens |
GOX | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | a second-order rate constant for the capture of glycolate (kcat/Kglycolate) of 68300 M/s, and a second-order rate constant for reaction of the reduced flavin with oxygen (kcat/Koxygen) of 24500 Ms | Homo sapiens | |
15.7 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
20 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
31.2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
54.1 | - |
flavin | pH 7.0, 30°C, flavin reduction | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH profiles, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | dependent on | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
280 | - |
chloride | pH 7.0, 25°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme is involved in primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |