Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structures of recombinant GO complexed with sulfate, glyoxylate, and 4-carboxy-5-dodecylsulfanyl-1,2,3-triazole (CDST), determined by X-ray crystallography are reported. A loop region (loop 4), is completely visible when the GO active site contains a small ligand. Lack of electron density for this loop in the GOCDST complex, mimicking a large substrate, indicates that a disordered to ordered transition occurs with substrate binding. Conformational flexibility of Trp110 is responsible for enabling GO to react with R-hydroxy acids. Movement of Trp110 disrupts a hydrogen-bonding network between Trp110, Leu191, Tyr134, and Tyr208. This loss indicates that active site movements are directly linked to changes in the conformation of loop 4 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-carboxy-5-dodecylsulfanyl-1,2,3-triazole | - |
Homo sapiens | |
glyoxylate | substrate inhibition at concentrations above 4 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2-oxo-octanoate | - |
Homo sapiens | |
0.141 | - |
glycolate | - |
Homo sapiens | |
2.2 | - |
glyoxylate | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UJM8 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA-column, gel filtration and ion-exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyoctanoate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | 2-oxo-octanoate + reduced 2,6-dichlorophenolindophenol | - |
r | |
glycolate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
glyoxylate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycolate oxidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
2-oxo-octanoate | - |
Homo sapiens | |
0.7 | - |
glyoxylate | - |
Homo sapiens | |
4.1 | - |
glycolate | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Homo sapiens |