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Literature summary for 1.1.3.13 extracted from

  • Heath, R.S.; Birmingham, W.R.; Thompson, M.P.; Taglieber, A.; Daviet, L.; Turner, N.J.
    An engineered alcohol oxidase for the oxidation of primary alcohols (2019), ChemBioChem, 20, 276-281 .
    View publication on PubMed
Search for more literature for 1.1.3.13

Protein Variants

Protein Variants Comment Organism
M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A/D250G/F253R/V355T/F357R/M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13, with a 20fold increased kcat compared to that of the wildtype enzyme. This variant enables the oxidation of 10 mM hexanol to hexanal in less than 24h with 100% conversion and catalyzes significantly improved oxidation of saturated, unsaturated, aliphatic, cyclic and benzylic alcohols Arthrobacter globiformis
S101A/V355T/F357R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A/V355T/F357R/M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
V355T/F357R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 1-Hexanol mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.43
-
 1-Hexanol mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.67
-
 1-Hexanol mutant S101A, 30°C, pH 8 Arthrobacter globiformis
0.84
-
 1-Hexanol mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
3.16
-
 1-Hexanol mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
3.49
-
 1-Hexanol mutant M359R, 30°C, pH 8 Arthrobacter globiformis
6.14
-
 1-Hexanol wild-type, 30°C, pH 8 Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8 cf. EC 1.1.3.17
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00015
-
 wild-type, substrate 1-propanol, 30°C, pH 8 Arthrobacter globiformis
0.0044
-
 mutant S101A/D250G/F253R/V355T/F357R/M359R, substrate 1-propanol, 30°C, pH 8 Arthrobacter globiformis
0.02
-
 wild-type, substrate 1-hexanol, 30°C, pH 8 Arthrobacter globiformis
0.57
-
 mutant S101A/D250G/F253R/V355T/F357R/M359R, substrate 1-hexanol, 30°C, pH 8 Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexanol + O2 + H2O
-
 Arthrobacter globiformis hexanal + H2O2
-
 ?
1-propanol + O2
-
 Arthrobacter globiformis propanal + H2O2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 wild-type, stable for at least 3 h Arthrobacter globiformis
32
-
 melting temperature of wild-type Arthrobacter globiformis
40
-
 mutant S101A/V355T/F357R/M359R, 3 h, 10% residual activity. Mutant S101A/D250G/F253R/V355T/F357R/M359R, 3 h, almost 50% residual activity Arthrobacter globiformis
52
-
 metling temperature of mutant S101A/D250G/F253R/V355T/F357R/M359R Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 1-Hexanol mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.037
-
 1-Hexanol mutant M359R, 30°C, pH 8 Arthrobacter globiformis
0.047
-
 1-Hexanol wild-type, 30°C, pH 8 Arthrobacter globiformis
0.098
-
 1-Hexanol mutant S101A, 30°C, pH 8 Arthrobacter globiformis
0.165
-
 1-Hexanol mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.168
-
 1-Hexanol mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.34
-
 1-Hexanol mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 1-Hexanol wild-type, 30°C, pH 8 Arthrobacter globiformis
0.01
-
 1-Hexanol mutant M359R, 30°C, pH 8 Arthrobacter globiformis
0.147
-
 1-Hexanol mutant S101A, 30°C, pH 8 Arthrobacter globiformis
0.312
-
 1-Hexanol mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.392
-
 1-Hexanol mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.408
-
 1-Hexanol mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.423
-
 1-Hexanol mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis