Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli DH5 alpha as His-tagged protein | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
1,4-benzoquinone | substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 | Phanerodontia chrysosporium | |
0.051 | - |
2,6-dichloroindophenol | substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.09 | - |
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical | substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.11 | - |
1,4-benzoquinone | substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.21 | - |
2-methoxy-1,4-benzoquinone | substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.29 | - |
ferricenium hexafluorophosphate | substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 | Phanerodontia chrysosporium | |
0.31 | - |
2-chloro-1,4-benzoquinone | substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.33 | - |
ferricenium hexafluorophosphate | substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.51 | - |
2-methyl-1,4-benzoquinone | substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.64 | - |
tetrafluoro-1,4-benzoquinone | substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.84 | - |
D-glucose | substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
1.22 | - |
O2 | substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
1.59 | - |
2,6-dimethyl-1,4-benzoquinone | substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
2.94 | - |
D-galactose | substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
3.43 | - |
ferricyanide | substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
20.9 | - |
D-xylose | substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
23.5 | - |
L-sorbose | substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
103 | - |
D-fructose | substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
one subunit of recombinant expressed protein | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q6QWR1 | - |
- |
Purification (Comment) | Organism |
---|---|
homogenized in a French press, one-step immobilized metal affinity chromatography, concentrated in 50mM potassium phosphate buffer | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
2,6-dichloroindophenol + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
2,6-dimethyl-1,4-benzoquinone + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
2-chloro-1,4-benzoquinone + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
2-methoxy-1,4-benzoquinone + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
D-fructose + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
D-galactose + 1,4-benzoquinone | - |
Phanerodontia chrysosporium | 2-dehydro-D-galactose + 1,4-hydroquinone | - |
? | |
D-galactose + O2 | - |
Phanerodontia chrysosporium | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
D-glucose + 2-methyl-1,4-benzoquinone | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + 2-methylhydroquinone | - |
r | |
D-glucose + ferricyanide | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + ferrocyanide | - |
r | |
D-glucose + O2 | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-xylose + O2 | - |
Phanerodontia chrysosporium | D-xylosone + H2O2 | - |
? | |
ferricenium hexafluorophosphate + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
L-sorbose + O2 | - |
Phanerodontia chrysosporium | 5-dehydro-D-fructose + H2O2 | - |
? | |
tetrafluoro-1,4-benzoquinone + O2 | - |
Phanerodontia chrysosporium | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | suggested by observed molecular weight | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
P2Ox | - |
Phanerodontia chrysosporium |
pyranose 2-Oxidase | - |
Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-methyl-1,4-benzoquinone | substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.15 | - |
2-chloro-1,4-benzoquinone | substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
0.3 | - |
ferricyanide | substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
1.33 | - |
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical | substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
1.88 | - |
D-fructose | substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
4.87 | - |
D-galactose | substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
6.61 | - |
tetrafluoro-1,4-benzoquinone | substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
8.69 | - |
2,6-dimethyl-1,4-benzoquinone | substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
40.1 | - |
2-methoxy-1,4-benzoquinone | substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
44.9 | - |
D-xylose | substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
58.8 | - |
L-sorbose | substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
83.1 | - |
D-glucose | substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
108 | - |
2,6-dichloroindophenol | substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
109 | - |
O2 | substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
228 | - |
ferricenium hexafluorophosphate | substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
400 | - |
1,4-benzoquinone | substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 | Phanerodontia chrysosporium | |
477 | - |
1,4-benzoquinone | substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 | Phanerodontia chrysosporium | |
549 | - |
ferricenium hexafluorophosphate | substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 | Phanerodontia chrysosporium |