Cloned (Comment) | Organism |
---|---|
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
(2R,3S)-butane-2,3-diol | pH 7.4, 30°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc binding enzyme | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | meso-butane-2,3-diol | (3R)-acetoin + NADH + H+ | - |
? | |
(R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | - |
(R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HWM8 | - |
- |
Pseudomonas aeruginosa 1C | Q9HWM8 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9HWM8 | - |
- |
Pseudomonas aeruginosa CIP 104116 | Q9HWM8 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HWM8 | - |
- |
Pseudomonas aeruginosa JCM 14847 | Q9HWM8 | - |
- |
Pseudomonas aeruginosa LMG 12228 | Q9HWM8 | - |
- |
Pseudomonas aeruginosa PRS 101 | Q9HWM8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.155 | - |
pH 7.4, 30°C, substrate propane-1,2-diol | Pseudomonas aeruginosa |
0.331 | - |
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone | Pseudomonas aeruginosa |
0.383 | - |
pH 7.4, 30°C, substrate butane-1,2-diol | Pseudomonas aeruginosa |
1.179 | - |
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol | Pseudomonas aeruginosa |
1.346 | - |
pH 7.4, 30°C, substrate 2,3-pentanedione | Pseudomonas aeruginosa |
1.538 | - |
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol | Pseudomonas aeruginosa |
1.949 | - |
pH 7.4, 30°C, substrate rac acetoin | Pseudomonas aeruginosa |
2.154 | - |
pH 7.4, 30°C, substrate diacetyl | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | meso-butane-2,3-diol | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
(2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
(R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
r | |
1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
2,3-pentandione + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
butane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
diacetyl + 2 NADH + 2 H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + 2 NAD+ | - |
r | |
diacetyl + NADH + H+ | - |
Pseudomonas aeruginosa | (3R)-acetoin + NAD+ | - |
r | |
additional information | the (2R,3R)-2,3-butanediol dehydrogenase is active with (R,R)-butane-2,3-diol, but also with meso-butane-2,3-diol, but not with with (2S,3S)-2,3-butane-2,3-diol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa | ? | - |
- |
|
propane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + NAD+ | - |
? | |
rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3S)-butane-2,3-diol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Pseudomonas aeruginosa |
More | cf. EC 1.1.1.303 | Pseudomonas aeruginosa |
PA4153 | locus name | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa | |
NADH | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
malfunction | the amount of meso-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The loss of locus pa4153, encoding (2R,3R)-2,3-BDH, has no effect on the ability of this strain to grow in (2S,3S)-2,3-BD but completely impairs its ability to utilize (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4153 mutant strain with its gene successfully restores the growth ability. The DELTApa4153 PAO1 strain can grow in racemic acetoin, indicating that (2R,3R)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin | Pseudomonas aeruginosa |
metabolism | 2,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon | Pseudomonas aeruginosa |
physiological function | 2,3-butanediol (2,3-BD) is a primary microbial metabolite that enhances the virulence of Pseudomonas aeruginosa and alters the lung microbiome. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD | Pseudomonas aeruginosa |