Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HPO42- | - |
Glycine max | |
SO42- | - |
Glycine max |
General Stability | Organism |
---|---|
dilution inactivates | Glycine max |
dithiothreitol increases stability | Glycine max |
inorganic ions, i.e. Cl-, SO42-, HPO42-, essential for stability | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | noncompetitive to phosphohydroxypyruvate | Glycine max | |
L-serine | not | Glycine max | |
NAD+ | product inhibition, competitive to NADH | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADH | pH 7.5 | Glycine max | |
0.15 | - |
NADPH | pH 7.5 | Glycine max | |
0.15 | - |
D-3-phosphoglycerate | pH 7.5 | Glycine max | |
0.25 | - |
NAD+ | pH 9.4 | Glycine max | |
0.29 | - |
D-3-phosphoglycerate | pH 9.4 | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | stimulates | Glycine max | |
MgCl2 | stimulates | Glycine max | |
SO42- | stimulates | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + NAD+ | Glycine max | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | 3-phosphohydroxypyruvate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.3 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NADP+ | - |
Glycine max | 3-phosphohydroxypyruvate + NADPH + H+ | - |
? | |
3-phosphoglycerate + NAD+ | - |
Glycine max | 3-phosphohydroxypyruvate + NADH | - |
r | |
3-phosphoglycerate + NAD+ | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | Glycine max | 3-phosphohydroxypyruvate + NADH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
NADH oxidation | Glycine max |
9.4 | - |
NAD+ reduction | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.8 | about 50% of activity maximum at pH 5 and 7.8, NADH oxidation | Glycine max |
8.5 | 10.3 | about 50% of activity maximum at pH 8.5 and 10.3, NAD+ reduction | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Glycine max | |
NADH | - |
Glycine max | |
NADPH | - |
Glycine max |