Cloned (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structure of the holoenzyme to 1.9 A resolution | Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
D38S/M39R | the mutant enzyme is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
NADP+ | pH 7.0, mutant enzyme D38S/M39R | Gluconobacter oxydans | |
0.348 | - |
NAD+ | pH 7.0, wild-type enzyme | Gluconobacter oxydans | |
13.7 | - |
xylitol | pH 7.0, cofactor: NAD+, wild-type enzyme | Gluconobacter oxydans | |
100 | - |
xylitol | pH 7.0, cofactor: NADP+ mutant enzyme D38S/M39R | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q8GR61 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
xylitol + NADP+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D38S/M39R is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans | D-xylulose + NADPH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.206 | - |
NADP+ | pH 7.0, mutant enzyme D38S/M39R | Gluconobacter oxydans | |
17.9 | - |
xylitol | pH 7.0, cofactor: NADP+mutant enzyme D38S/M39R | Gluconobacter oxydans | |
24.6 | - |
xylitol | pH 7.0, cofactor: NAD+, wild-type enzyme | Gluconobacter oxydans | |
27.2 | - |
NAD+ | pH 7.0, wild-type enzyme | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ specificity is largely conferred by Asp38, which interacts with the hydroxyls of the adenosine ribose | Gluconobacter oxydans | |
NADP+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D38S/M39R is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans |