Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | isoenzyme 2, 1 mM, 87% inhibition, isoenzyme 1, 1 mM, 17% inhibition | Saccharomyces cerevisiae | |
iodoacetate | isoenzyme 2, 1 mM, 97% inhibition, isoenzyme 1, 1 mM, 18% inhibition | Saccharomyces cerevisiae | |
N-ethylmaleimide | 1 mM, 58% inhibition | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | isoenzyme 2, 1 mM, complete inhibition, 0.1 mM, 88% inhibition, 29% inhibition in the presence of 1 mM dithiothreitol, 21% inhibition in the presence of 1 mM L-cysteine, isoenzyme 1, 1 mM, 59% inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
NADPH | - |
Saccharomyces cerevisiae | |
0.43 | - |
NADH | - |
Saccharomyces cerevisiae | |
1.4 | - |
Hydroxypyruvate | - |
Saccharomyces cerevisiae | |
16 | - |
glyoxylate | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | Saccharomyces cerevisiae | - |
glycolate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150, phosphocellulose | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.7 | - |
isoenzyme 2 | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
isoenzyme 2, 4°C, 10 mM, potassium phosphate, pH 7.0, 20% glycerol, 1 week, 10% loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | - |
Saccharomyces cerevisiae | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | 7% of activity with glyoxylate | Saccharomyces cerevisiae | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NADH + H+ | - |
Saccharomyces cerevisiae | glycolate + NAD+ | - |
r | |
hydroxypyruvate + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | glycerate + NAD(P)+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoenzyme 2, 90% loss of activity after 5 min, 20% glycerol protects from inactivation, enzyme retains 65% of its activity after 5 min at 40°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | reduction of hydroxypyruvate | Saccharomyces cerevisiae |
6 | 6.3 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Saccharomyces cerevisiae |