Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 123% activity at 1 mM | Ogataea wickerhamii | |
N-ethylmaleimide | 147% activity at 1 mM | Ogataea wickerhamii | |
o-phenanthroline | 123% activity at 1 mM | Ogataea wickerhamii | |
p-chloromercuribenzoate | 118% activity at 0.05 mM | Ogataea wickerhamii | |
phenylmethylsulfonyl fluoride | 111% activity at 1 mM | Ogataea wickerhamii | |
ZnSO4 | 152% activity at 1 mM | Ogataea wickerhamii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Ogataea wickerhamii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 80.6% residual activity at 1 mM | Ogataea wickerhamii | |
Fe2+ | 93.2% residual activity at 1 mM | Ogataea wickerhamii | |
Hg+ | 61.9% residual activity at 1 mM | Ogataea wickerhamii | |
additional information | not inhibited by Co2+, Zn2+, K+, Ni2+, Ba2+, NH2OH, and quercetin | Ogataea wickerhamii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 174% activity at 1 mM | Ogataea wickerhamii | |
Cu2+ | 100% activity at 1 mM | Ogataea wickerhamii | |
Mg2+ | 162% activity at 1 mM | Ogataea wickerhamii | |
Mn2+ | 185% activity at 1 mM | Ogataea wickerhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Ogataea wickerhamii |
83000 | - |
gel filtration | Ogataea wickerhamii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea wickerhamii | A0JC04 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, HiTrap Blue column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Ogataea wickerhamii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0224 | - |
crude extract, at pH 8.5 and 30°C | Ogataea wickerhamii |
91.1 | - |
after 4070fold purification, at pH 8.5 and 30°C | Ogataea wickerhamii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-1-phenylethan-1-ol + NAD+ | 255% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | phenylacetaldehyde + NADH + H+ | - |
? | |
(1S)-1-phenylethan-1-ol + NAD+ | 1.8% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | phenylacetaldehyde + NADH + H+ | - |
? | |
(2R)-butan-2-ol + NAD+ | 49.3% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | butan-2-one + NADH + H+ | - |
? | |
(2R)-hexan-2-ol + NAD+ | 54.1% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | hexan-2-one + NADH + H+ | - |
? | |
(2S)-butan-2-ol + NAD+ | 7.3% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | butan-2-one + NADH + H+ | - |
? | |
(R)-octan-2-ol + NAD+ | 100% activity | Ogataea wickerhamii | octan-2-one + NADH + H+ | - |
? | |
(RS)-octan-2-ol + NAD+ | 77.8% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | octan-2-one + NADH + H+ | - |
? | |
(S)-octan-2-ol + NAD+ | 4.7% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | octan-2-one + NADH + H+ | - |
? | |
1-phenoxypropan-2-ol + NAD+ | 15.5% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | 1-phenoxypropan-2-one + NADH + H+ + | - |
? | |
1-phenoxypropan-2-one + NADH + H+ | 54.2% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 1-phenoxypropan-2-ol + NAD+ | - |
? | |
1-phenylethan-1-one + NADH + H+ | 45.3% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 1-phenylethan-1-ol + NAD+ | - |
? | |
2-bromo-1-cyclopropylethan-1-one + NADH + H+ | 54.3% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 2-bromo-1-cyclopropylethan-1-ol + NAD+ | - |
? | |
2-chloro-1-(3-chlorophenyl)ethan-1-one + NADH + H+ | 64.7% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 2-chloro-1-(3-chlorophenyl)ethan-1-ol + NAD+ | - |
? | |
2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+ | 89.2% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+ | - |
? | |
2-chloro-1-(pyridin-3-yl)ethan-1-one + NADH + H+ | 9.4% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 2-chloro-1-(pyridin-3-yl)ethan-1-ol + NAD+ | - |
? | |
2-chloro-1-phenylethan-1-one + NADH + H+ | 52.6% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 2-chloro-1-phenylethan-1-ol + NAD+ | - |
? | |
3-acetyloxolan-2-one + NADH + H+ | 12.7% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 3-acetyloxolan-2-ol + NAD+ | - |
? | |
5-chloropentan-2-one + NADH + H+ | 108% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | 5-chloropentan-2-ol + NAD+ | - |
? | |
butan-2-one + NADH + H+ | 8.0% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | butan-2-ol + NAD+ | - |
? | |
ethanol + NAD+ | 0.7% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | acetaldehyde + NADH + H+ | - |
? | |
ethyl (3R)-3-hydroxybutanoate + NAD+ | 41.1% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | ethyl 3-oxobutanoate + NADH + H+ | - |
? | |
ethyl (3S)-3-hydroxybutanoate + NAD+ | 1.0% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | ethyl 3-oxobutanoate + NADH + H+ | - |
? | |
ethyl (3S)-4-chloro-3-hydroxybutanoate + NAD+ | 2.2% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | ethyl 4-chloro-3-oxobutanoate + NADH + H+ | - |
? | |
ethyl 2-chloro-3-oxobutanoate + NADH + H+ | 571% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | ethyl 2-chloro-3-oxobutanoate + NAD+ | - |
? | |
ethyl 3-oxobutanoate + NADH + H+ | 105.8% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | ethyl 3-hydroxybutanoate + NAD+ | - |
? | |
ethyl 4-chloroacetoacetate + NADH + H+ | 100% activity | Ogataea wickerhamii | ? | - |
? | |
octan-1-ol + NAD+ | 1.7% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | octanal + NADH + H+ | - |
? | |
octan-2-one + NADH + H+ | 31.2% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | octan-2-ol + NAD+ | - |
? | |
propan-2-ol + NAD+ | 6.8% activity compared to (R)-octan-2-ol | Ogataea wickerhamii | propan-2-one + NADH + H+ | - |
? | |
propan-2-one + NADH + H+ | 1.8% activity compared to ethyl 4-chloroacetoacetate | Ogataea wickerhamii | propan-2-ol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 2 * 30000, SDS-PAGE | Ogataea wickerhamii |
Synonyms | Comment | Organism |
---|---|---|
(R)-2-octanol dehydrogenase | - |
Ogataea wickerhamii |
ODH | - |
Ogataea wickerhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
oxidation reaction | Ogataea wickerhamii |
50 | 55 | reduction reaction | Ogataea wickerhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction reaction | Ogataea wickerhamii |
10.5 | - |
oxidation reaction | Ogataea wickerhamii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ogataea wickerhamii |