Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21(DE3) strain | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, about 40% loss of activity | Pseudomonas putida | |
Cu2+ | 5 mM, about 70% loss of activity | Pseudomonas putida | |
Zn2+ | 5 mM, about 80% loss of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
17beta-estradiol | pH 9.0, 37°C | Pseudomonas putida | |
0.75 | - |
estrone | pH 9.0, 37°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | weak stimulation | Pseudomonas putida | |
Mn2+ | weak stimulation | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NAD+ | Pseudomonas putida | - |
estrone + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | Pseudomonas putida SJTE-1 | - |
estrone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | ANI02794.1 | - |
- |
Pseudomonas putida SJTE-1 | ANI02794.1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NAD+ | - |
Pseudomonas putida | estrone + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | transformation efficiency of 17beta-estradiol into estrone is over 96.6% in five minutes | Pseudomonas putida | estrone + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | - |
Pseudomonas putida SJTE-1 | estrone + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | transformation efficiency of 17beta-estradiol into estrone is over 96.6% in five minutes | Pseudomonas putida SJTE-1 | estrone + NADH + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 35% of maximal activity, 50°C: about 40% of maximal activity | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
estrone | pH 9.0, 37°C | Pseudomonas putida | |
2.1 | - |
17beta-estradiol | pH 9.0, 37°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pH 6.0: about 30% of maximal activity, pH 11.0: about 40% of maximal activity | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity with NADP+ | Pseudomonas putida | |
NADH | no activity with NADPH | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays an important role in estrogen metabolism | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.19 | - |
estrone | pH 9.0, 37°C | Pseudomonas putida | |
25.61 | - |
17beta-estradiol | pH 9.0, 37°C | Pseudomonas putida |