Protein Variants | Comment | Organism |
---|---|---|
K18A | the mutant with reduced catalytic efficiency shows 35fold preference for NADP+ over NAD+ as compared to the wild type enzyme (386fold) | Escherichia coli |
K18A/R50A | the mutant shows almost no activity | Escherichia coli |
K18T | the mutant with reduced catalytic efficiency sshows 35fold preference for NADP+ over NAD+ as compared to the wild type enzyme (386fold) | Escherichia coli |
R50A | the mutant with reduced catalytic efficiency shows 50fold preference for NADP+ over NAD+ as compared to the wild type enzyme (386fold) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
NADP+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli | |
0.099 | - |
NADP+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
0.382 | - |
NADP+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
2.477 | - |
NAD+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
5.09 | - |
NAD+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli | |
11.74 | - |
NAD+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli | |
14.66 | - |
NAD+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
17.7 | - |
NADP+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Escherichia coli | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | very low activity with NAD+ | Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Escherichia coli |
glucose-6-phosphate dehydrogenase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
109 | - |
NAD+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
143 | - |
NADP+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
165 | - |
NAD+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli | |
171 | - |
NAD+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
174 | - |
NADP+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli | |
185 | - |
NADP+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli | |
189 | - |
NADP+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
288 | - |
NAD+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme has strong preference towards NADP+ | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NAD+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli | |
0.001 | - |
NADP+ | mutant enzyme K18A/R50A, at pH 8.2 and 25°C | Escherichia coli | |
0.01 | - |
NAD+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
0.04 | - |
NAD+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
0.06 | - |
NAD+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli | |
500 | - |
NADP+ | mutant enzyme R50A, at pH 8.2 and 25°C | Escherichia coli | |
1400 | - |
NADP+ | mutant enzyme K18A, at pH 8.2 and 25°C | Escherichia coli | |
23200 | - |
NADP+ | wild type enzyme, at pH 8.2 and 25°C | Escherichia coli |