Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in the G6PD-deficient Escherichia coli strain DF213 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | G6PD mutations are thought to cause haemolytic anaemia by compromising enzyme stability, phenotypes | Homo sapiens |
R454C | site-directed mutagenesis, the mutant strain overexpresses the clinical enzyme mutants, i.e. Union clone, C1360T, the mutation abolishes a salt bridge between Arg454 and Asp 286, and leads to 10% decreased kcat and activity, Km values for both G6P and NADP+ are decreased approximately 5fold, the mutant shows decreased thermostability | Homo sapiens |
R454H | site-directed mutagenesis, the mutant strain overexpresses the clinical enzyme mutants, i.e. Andalus clone, G1361A, the mutation abolishes a salt bridge between Arg454 and Asp 286, and leads to 10% decreased kcat and activity, Km values for both G6P and NADP+ are decreased approximately 5fold, the mutant shows decreased thermostability | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of wild-type and mutant enzymes | Homo sapiens | |
0.00238 | - |
NADP+ | pH 8.0, 25°C, recombinant mutant R454H, with D-glucose 6-phosphate | Homo sapiens | |
0.00276 | - |
NADP+ | pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate | Homo sapiens | |
0.00707 | - |
NADP+ | pH 8.0, 25°C, recombinant wild-type enzyme, with D-glucose 6-phosphate | Homo sapiens | |
0.00839 | - |
NADP+ | pH 8.0, 25°C, recombinant mutant R454C, with deoxy-D-glucose 6-phosphate | Homo sapiens | |
0.00953 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with NADP+ | Homo sapiens | |
0.00971 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454H, with NADP+ | Homo sapiens | |
0.0156 | - |
deamino-NADP+ | pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate | Homo sapiens | |
0.0488 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ | Homo sapiens | |
0.052 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+ | Homo sapiens | |
0.374 | - |
2-deoxy-D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with NADP+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Homo sapiens | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
X-linked house-keeping enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from G6PD-deficient Escherichia coli strain DF213 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | rapid-equilibrium random-order mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | ? | - |
? | |
D-glucose 6-phosphate + deamino-NADP+ | - |
Homo sapiens | ? | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Homo sapiens |
glucose 6-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at physiological temperatures the mutant enzymes looses only 10% of their activity after 20 min in the presence of 0.01 mM NADP+ | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | 50 | D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ | Homo sapiens | |
22.4 | - |
2-deoxy-D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with NADP+ | Homo sapiens | |
28.6 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with NADP+ | Homo sapiens | |
39.9 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454H, with NADP+ | Homo sapiens | |
87.7 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ | Homo sapiens | |
275 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+ | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |