Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thauera aromatica |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M Mg (CH3COO)2, 0.1 M sodium cacodylate, pH 6.5, and 20% (w/v) PEG 8000 | Thauera aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
(S)-2-benzoylsuccinyl-CoA | at pH 6.2 and 30°C | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-benzoylsuccinyl-CoA + NADH + H+ | Thauera aromatica | - |
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and hydroxyapatite column chromatography | Thauera aromatica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
crude extract, at pH 6.2 and 30°C | Thauera aromatica |
0.86 | - |
after 3.1 enrichment, at pH 6.2 and 30°C | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-benzoylsuccinyl-CoA + NADH + H+ | - |
Thauera aromatica | (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+ | - |
? | |
additional information | the enzyme does not use NADP+ as electron acceptor | Thauera aromatica | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 26000 + 2 * 28000, SDS-PAGE | Thauera aromatica |
Synonyms | Comment | Organism |
---|---|---|
(S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase | - |
Thauera aromatica |
bbsCD | - |
Thauera aromatica |