Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | when assayed at malate concentrations of 0.2 mM, fumarate activates the enzyme by 80% over the control activity | Zea mays | |
additional information | after PEG 6000 treatment, the levels of cytosolic NADP-ME transcripts are enhanced by approximately 2.5times | Zea mays | |
succinate | when assayed at malate concentrations of 0.2 mM, succinate activates the enzyme by 121% over the control activity, at malate concentration of 1.2 mM, succinate activates the enzyme by 62% over the control activity | Zea mays |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1,6-bisphosphate | when assayed at malate concentrations of 0.2 mM, D-fructose-1,6-bisphosphate inhibits the enzyme by a 49% over the control activity | Zea mays | |
additional information | cytosolic NADP-ME expression in roots decreases with development, decreased levels of expression of cytosolic NADP-ME is observed in roots after incubating in solutions of Na2CO3 at pH 11.0 or NaHCO3 at pH 6.5; cytosolic NADP-ME is not inhibited by high malate concentrations at pH 7.0; NADP-ME is not affected by acetyl-CoA, CoA, pyruvate, L-alanine, alpha-ketoglutarate, glycerol-3-phosphate, 3-phospho-glycerate, and citrate | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Zea mays | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
mature monomer of recombinant enzyme after enterokinase digestion, SDS-PAGE | Zea mays |
71600 | - |
calculated from amino acid sequence | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q5G1U0 | inbred line AX882 (Nidera) | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Zea mays | - |
root | emerging root | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer or octamer | - |
Zea mays |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Zea mays |
NADP-ME | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Zea mays |