Protein Variants | Comment | Organism |
---|---|---|
additional information | isozyme Mdh1 overexpression in yeast extends life span of the organism requiring respiration and the Sir2 family, while the mdh1DELTAaat1DELTA double mutation blocks CR-mediated life span extension and also prevents the characteristic decrease in the NADH levels in the cytosolic/nuclear pool, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | Mdh2 | Saccharomyces cerevisiae | 5737 | - |
mitochondrion | Mdh1, matrix | Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Saccharomyces cerevisiae | - |
oxaloacetate + NADH + H+ | - |
r | |
additional information | Saccharomyces cerevisiae | the malate-aspartate NADH shuttle components are metabolic longevity regulators required for calorie restriction-mediated life span extension in yeast, the shuttle plays a major role in the activation of the downstream targets of calorie restriction such as Sir2, mechanism and regulation, detailed overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
This UniProt-ID has been deleted; isozyme Mdh1 | - |
Saccharomyces cerevisiae | P22133 | Mdh2; isozyme Mdh2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Saccharomyces cerevisiae | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | mitochondrial isozyme Mdh1 preferably catalyzes the oxaloacetate formation, overview | Saccharomyces cerevisiae | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | the cytoplasmic isozyme Mdh2 catalyzes preferably the malate formation, overview | Saccharomyces cerevisiae | oxaloacetate + NADH + H+ | - |
r | |
additional information | the malate-aspartate NADH shuttle components are metabolic longevity regulators required for calorie restriction-mediated life span extension in yeast, the shuttle plays a major role in the activation of the downstream targets of calorie restriction such as Sir2, mechanism and regulation, detailed overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malate dehydrogenase | - |
Saccharomyces cerevisiae |
Mdh1 | - |
Saccharomyces cerevisiae |
MDH2 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Saccharomyces cerevisiae | |
NADH | dependent on | Saccharomyces cerevisiae |