Protein Variants | Comment | Organism |
---|---|---|
D205C | enzyme variant with cysteine close to the dimer interface, about 30% loss of specific activity compared to wild-type enzyme shows changes in activity and the efficacy of immobilization. | Leuconostoc mesenteroides |
D453C | enzyme variant with cysteine far from the active center, no significant loss in specific activity compared to wild-type enzyme, in contrast to wild-type enzyme, the mutant enzyme is readily immobilited | Leuconostoc mesenteroides |
L218C | enzyme variant with cysteine close to the active center, about 30% loss of specific activity compared to wild-type enzyme, the mutant enzyme shows almost complete immobilization but poor carrier activity | Leuconostoc mesenteroides |
General Stability | Organism |
---|---|
eluted mutant enzyme D453C shows almost double the activity of the immobilized enzyme, which is consistent with 49% activity loss due to immobilization. Mutant enzyme D205C produces a 1.8-fold higher activity compared to its immobilized state. Eluted mutant enzyme L218C shows 9.9 times the activity of its immobilized state | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, SDS-PAGE | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 8 months, enzym variants L218C, D205C or D453C, no loss of activity | Leuconostoc mesenteroides |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leuconostoc mesenteroides |