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show all sequences of 1.1.1.346

Purification and characterization of the 2-ketoaldonate reductase from Brevibacterium ketosoreductum ATCC21914

Yum, D-Y.; Bae, S-S.; Pan, J-G.; Biosci. Biotechnol. Biochem. 62, 154-156 (1998)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
at pH 6.4 and 30C
Brevibacterium ketosoreductum
5
-
2,5-didehydro-D-gluconate
at pH 6.4 and 30C
Brevibacterium ketosoreductum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
72000
-
gel filtration
Brevibacterium ketosoreductum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevibacterium ketosoreductum
-
-
-
Brevibacterium ketosoreductum ATCC 21914
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration
Brevibacterium ketosoreductum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.07
-
crude extract, at pH 6.4 and 30C
Brevibacterium ketosoreductum
22.2
-
after 317fold purification, at pH 6.4 and 30C
Brevibacterium ketosoreductum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-didehydro-D-gluconate + NADPH + H+
100% activity
286278
Brevibacterium ketosoreductum
5-dehydro-D-gluconate + NADP+
-
-
-
?
2-dehydro-D-gluconate + NADPH + H+
11.8% activity compared to 2,5-didehydro-D-gluconate
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
-
-
?
2-dehydro-L-gulonate + NADPH + H+
10.8% activity compared to 2,5-didehydro-D-gluconate
286278
Brevibacterium ketosoreductum
L-idonate + NADP+
-
-
-
?
additional information
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
286278
Brevibacterium ketosoreductum
?
-
-
-
-
additional information
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
286278
Brevibacterium ketosoreductum ATCC 21914
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Brevibacterium ketosoreductum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.2
10
after 2 h incubation at 30C, the remaining activity is about 35% of the original level at pH 8.4, and no remaining activity is observed below pH 5.1 and 10.0. The enzyme is stable from pH 6.0 to 7.5
Brevibacterium ketosoreductum
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Brevibacterium ketosoreductum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Brevibacterium ketosoreductum
isoelectric focusing
-
4.7
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Brevibacterium ketosoreductum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
at pH 6.4 and 30C
Brevibacterium ketosoreductum
5
-
2,5-didehydro-D-gluconate
at pH 6.4 and 30C
Brevibacterium ketosoreductum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
72000
-
gel filtration
Brevibacterium ketosoreductum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration
Brevibacterium ketosoreductum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.07
-
crude extract, at pH 6.4 and 30C
Brevibacterium ketosoreductum
22.2
-
after 317fold purification, at pH 6.4 and 30C
Brevibacterium ketosoreductum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-didehydro-D-gluconate + NADPH + H+
100% activity
286278
Brevibacterium ketosoreductum
5-dehydro-D-gluconate + NADP+
-
-
-
?
2-dehydro-D-gluconate + NADPH + H+
11.8% activity compared to 2,5-didehydro-D-gluconate
286278
Brevibacterium ketosoreductum
D-gluconate + NADP+
-
-
-
?
2-dehydro-L-gulonate + NADPH + H+
10.8% activity compared to 2,5-didehydro-D-gluconate
286278
Brevibacterium ketosoreductum
L-idonate + NADP+
-
-
-
?
additional information
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
286278
Brevibacterium ketosoreductum
?
-
-
-
-
additional information
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
286278
Brevibacterium ketosoreductum ATCC 21914
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 35000, SDS-PAGE
Brevibacterium ketosoreductum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Brevibacterium ketosoreductum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.2
10
after 2 h incubation at 30C, the remaining activity is about 35% of the original level at pH 8.4, and no remaining activity is observed below pH 5.1 and 10.0. The enzyme is stable from pH 6.0 to 7.5
Brevibacterium ketosoreductum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Brevibacterium ketosoreductum
isoelectric focusing
-
4.7
Other publictions for EC 1.1.1.346
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742259
Chen
Cloning, expression and chara ...
Comamonas testosteroni, Comamonas testosteroni ATCC 11996
Chem. Biol. Interact.
234
229-235
2015
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1
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1
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4
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2
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1
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4
1
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1
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1
1
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1
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4
-
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-
1
-
-
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-
4
1
-
-
-
-
-
-
-
-
1
3
3
1
-
-
741575
Kaswurm
Evaluation of the food grade ...
Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20301
AMB Express
3
7-17
2013
-
1
1
-
1
-
-
-
-
-
-
2
-
2
-
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6
-
2
1
1
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1
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2
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1
1
2
-
1
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2
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6
-
2
1
1
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-
-
1
-
-
-
-
2
2
-
-
-
657308
Sanli
Structural alteration of cofac ...
Corynebacterium sp.
Protein Sci.
13
504-512
2004
-
-
-
1
1
-
-
-
-
-
-
-
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1
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1
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1
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1
1
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440310
Banta
Alteration of the specificity ...
Corynebacterium sp.
Protein Eng.
15
131-140
2002
-
-
1
-
40
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
40
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440311
Banta
Optimizing an artificial metab ...
Corynebacterium sp.
Biochemistry
41
6226-6236
2002
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
5
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-
-
1
-
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1
-
5
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-
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-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
440312
Habrych
Purification and identificatio ...
Escherichia coli, Escherichia coli BL21-(DE3)
Biotechnol. Prog.
18
257-261
2002
-
-
-
-
-
-
-
1
-
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1
-
-
2
-
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1
-
-
-
1
1
5
1
-
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-
1
-
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1
-
1
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-
1
-
-
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1
-
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1
-
-
-
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1
-
-
1
1
5
1
-
-
-
1
-
-
-
1
-
1
1
-
-
-
440307
Khurana
Molecular modeling of substrat ...
Corynebacterium sp.
Proteins
39
68-75
2000
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
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1
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1
-
2
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-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
440306
Yum
Identification of the yqhE and ...
Escherichia coli
Appl. Environ. Microbiol.
65
3341-3346
1999
-
-
-
-
-
-
-
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3
-
-
8
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1
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2
1
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1
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-
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3
-
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1
-
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-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum, Brevibacterium ketosoreductum ATCC 21914
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
-
-
2
-
-
1
-
-
-
2
-
5
1
-
-
-
-
1
-
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
2
-
5
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
440305
Khurana
Crystal structure of 2,5-diket ...
Corynebacterium sp.
Proc. Natl. Acad. Sci. USA
95
6768-6773
1998
-
-
1
1
-
-
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3
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1
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-
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1
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1
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1
1
1
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1
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1
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-
-
-
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-
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-
721898
Maremonti
-
Characterisation of 2,5-diketo ...
Corynebacterium sp.
Biotechnol. Lett.
18
845-850
1996
-
-
-
-
-
-
-
1
-
-
2
-
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1
-
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1
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2
-
1
1
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1
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1
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1
-
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2
-
-
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1
-
-
2
-
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
440303
Sonoyama
-
Purification and properties of ...
Corynebacterium sp., Corynebacterium sp. SHS 0007
J. Ferment. Technol.
65
311-317
1987
-
-
-
-
-
-
-
4
-
-
2
-
-
2
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1
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5
1
1
-
1
-
2
-
1
1
-
2
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-
1
-
-
-
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-
4
-
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2
-
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1
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-
-
-
5
1
1
-
1
-
2
-
1
2
-
-
-
-
-
-
440304
Miller
Purification and characterizat ...
Corynebacterium sp., Corynebacterium sp. ATCC 31090
J. Biol. Chem.
262
9016-9020
1987
-
-
-
-
-
-
6
6
1
-
2
-
-
2
-
-
1
-
-
-
2
2
5
1
-
-
-
1
2
1
-
1
1
1
-
-
-
-
1
-
-
-
-
6
1
6
1
-
2
-
-
-
-
1
-
-
2
2
5
1
-
-
-
1
2
1
-
1
-
-
-
-
-
-