Literature summary for 1.1.1.343 extracted from

Sarmiento-Pavia, P.D.; Rodriguez-Hernandez, A.; Rodriguez-Romero, A.; Sosa-Torres, M.E.
The structure of a novel membrane-associated 6-phosphogluconate dehydrogenase from Gluconacetobacter diazotrophicus (Gd6PGD) reveals a subfamily of short-chain 6PGDs (2021), FEBS J., 288, 1286-1304 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method at 18°C	Gluconacetobacter diazotrophicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0334	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
0.0529	-	NAD+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
0.103	-	NAD+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
0.869	-	NADP+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
0.934	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
2.393	-	NADP+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	membrane-associated. The enzyme is located in both the cytoplasm and membrane	Gluconacetobacter diazotrophicus	5737	-
membrane	membrane-associated. The enzyme is located in both the cytoplasm and membrane	Gluconacetobacter diazotrophicus	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	non-denaturing PAGE	Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-phospho-D-gluconate + NAD+	Gluconacetobacter diazotrophicus	the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?
6-phospho-D-gluconate + NAD+	Gluconacetobacter diazotrophicus ATCC 49037	the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Gluconacetobacter diazotrophicus	A9H324	-	-
Gluconacetobacter diazotrophicus ATCC 49037	A9H324	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-phospho-D-gluconate + NAD+	the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis	Gluconacetobacter diazotrophicus	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?
6-phospho-D-gluconate + NAD+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?
6-phospho-D-gluconate + NAD+	the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis	Gluconacetobacter diazotrophicus ATCC 49037	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?
6-phospho-D-gluconate + NAD+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus ATCC 49037	D-ribulose 5-phosphate + CO2 + NADH + H+	-	?
6-phospho-D-gluconate + NADP+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	?
6-phospho-D-gluconate + NADP+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus ATCC 49037	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 35000, SDS-PAGE	Gluconacetobacter diazotrophicus

Synonyms

Synonyms	Comment	Organism
6-phosphogluconate dehydrogenase	-	Gluconacetobacter diazotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.97	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
1.74	-	NADP+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
2.62	-	NAD+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
2.8	-	NADP+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
3.63	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
6.06	-	NAD+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus
NADP+	the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+	Gluconacetobacter diazotrophicus

General Information

General Information	Comment	Organism
physiological function	the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis	Gluconacetobacter diazotrophicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.2	-	NADP+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
2	-	NADP+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
3.9	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus
29	-	6-phospho-D-gluconate	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
49	-	NAD+	pH 7.5, temperature not specified in the publication, membrane-purified enzyme	Gluconacetobacter diazotrophicus
59	-	NAD+	pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme	Gluconacetobacter diazotrophicus

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Release 2023.1 (January 2023)