Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Paracoccus denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
alpha4, 4 * 32000, SDS-PAGE | Paracoccus denitrificans |
132000 | - |
gel filtration | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Paracoccus denitrificans | - |
acetoacetate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
enzyme shows hysteresis, lag phase in progress curve | - |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing | Paracoccus denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97 | - |
- |
Paracoccus denitrificans |
Storage Stability | Organism |
---|---|
-20°C, several months, 0%, 4°C, lyophilized, 2 month, 0% inactivation, 4°C, 0.1 M sodium phosphate buffer pH 7-8, 5 days, 50% inactivation, stability increases in the presence of 1-10 mM EDTA | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Paracoccus denitrificans | acetoacetate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 32000, SDS-PAGE | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Paracoccus denitrificans |