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Literature summary for 1.1.1.283 extracted from

  • Lee, H.M.; Seo, J.H.; Kwak, M.K.; Kang, S.O.
    Methylglyoxal upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity (2017), Int. J. Biochem. Cell Biol., 90, 81-92 .
    View publication on PubMed
Search for more literature for 1.1.1.283

Activating Compound

Activating Compound Comment Organism Structure
methylglyoxal exogenous methylglyoxal (MG) upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Dictyostelium discoideum 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactaldehyde + NADP+ Dictyostelium discoideum
-
 methylglyoxal + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q6IMN8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information methylglyoxal (MG) level significantly increases and ROS level is maintained at high levels after mound stage Dictyostelium discoideum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactaldehyde + NADP+
-
 Dictyostelium discoideum methylglyoxal + NADPH + H+
-
 ?
additional information methylglyoxal-specific aldolase reductase activity Dictyostelium discoideum ?
-
-

Synonyms

Synonyms Comment Organism
AlrA
-
 Dictyostelium discoideum
MG-specific aldolase reductase
-
 Dictyostelium discoideum
More cf. EC 1.1.1.21 Dictyostelium discoideum
NADPH-linked aldolase reductase
-
 Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Dictyostelium discoideum

General Information

General Information Comment Organism
malfunction glutathione (GSH)-deprived Dictyostelium discoideum accumulates methylglyoxal (MG) and reactive oxygen species (ROS) during vegetative growth. MG increases after the mound stage in this strain, with a 2.6fold increase compared to early developmental stages. gamma-Glutamylcysteine synthetase overexpressing (gcsAOE) slugs trigger glutahione reductase (Gsr) and aldolase reductase activity to detoxify MG, while superoxide dismutase overexpressing (sod2OE) and catalase overexpressing (catAOE) slugs mainly decrease cellular ROS levels. The MG-specific activity of NADPH-linked aldolase reductase shows a noticeable increase in the gcsAOE slugs, indicating enhanced MG-scavenging reductase activity in gcsAOE slugs. In contrast to the increase observed in migrating sod2OE and catAOE slugs by treatment with MG and H2O2, the migration of gcsAOE slugs appeas unaffected. This behavior is caused by MG-triggered Gsr and NADPH-linked aldolase reductase activity, suggesting that GSH biosynthesis in gcsAOE slugs is specifically used for MG-scavenging activity Dictyostelium discoideum
physiological function methylglyoxal (MG) upregulates slug migration via MG-scavenging-mediated differentiation Dictyostelium discoideum