Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
Fe3+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
NADP+ | competitive product inhibition | Corynebacterium sp. | |
Ni2+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
Zn2+ | 0.5 mM, strong inhibition | Corynebacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | - |
Corynebacterium sp. | |
2.2 | - |
glycerol | - |
Corynebacterium sp. | |
26 | - |
2,5-didehydro-D-gluconate | - |
Corynebacterium sp. | |
155 | - |
5-keto-D-fructose | - |
Corynebacterium sp. | |
160 | - |
dihydroxyacetone | - |
Corynebacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29992 | - |
1 * 29992, deduced from nucleotide sequence | Corynebacterium sp. |
34000 | - |
1 * 34000, SDS-PAGE | Corynebacterium sp. |
35000 | - |
gel filtration | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, Cibacron blue F3GA agarose, gel filtration | Corynebacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.38 | - |
- |
Corynebacterium sp. |
Storage Stability | Organism |
---|---|
-70°C, 20 mM Tris-HCl, pH 7.5, over 6 months, no loss of activity | Corynebacterium sp. |
4°C, pH 6.5-7.5, at least 2 months, no loss of activity | Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADH | 170fold lower reduction rate with NADH compared to NADPH | Corynebacterium sp. | 2-keto-L-gulonate + NAD+ | - |
? | |
2,5-didehydro-D-gluconate + NADH | 170fold lower reduction rate with NADH compared to NADPH | Corynebacterium sp. ATCC 31090 | 2-keto-L-gulonate + NAD+ | - |
? | |
2,5-didehydro-D-gluconate + NADPH | stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate | Corynebacterium sp. | 2-keto-L-gulonate + NADP+ | - |
r | |
2,5-didehydro-D-gluconate + NADPH | stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate | Corynebacterium sp. ATCC 31090 | 2-keto-L-gulonate + NADP+ | - |
r | |
5-keto-D-fructose + NADPH | - |
Corynebacterium sp. | L-sorbose + NADP+ | - |
? | |
5-keto-D-fructose + NADPH | - |
Corynebacterium sp. ATCC 31090 | L-sorbose + NADP+ | - |
? | |
dihydroxyacetone + NADPH | - |
Corynebacterium sp. | glycerol + NADP+ | - |
? | |
dihydroxyacetone + NADPH | - |
Corynebacterium sp. ATCC 31090 | glycerol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Corynebacterium sp. |
monomer | 1 * 29992, deduced from nucleotide sequence | Corynebacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.33 | - |
2,5-didehydro-D-gluconate | - |
Corynebacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
9.2 | - |
oxidation of 2-keto-L-gulonate | Corynebacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.3 | reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
8 | 10.3 | oxidation of 2-keto-L-gulonate | Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Corynebacterium sp. | |
NADPH | - |
Corynebacterium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NADP+ | - |
Corynebacterium sp. |