Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | D-carnitine dehydrogenase, 1 mM, complete inactivation | Agrobacterium sp. 525a | |
Ag+ | D-carnitine dehydrogenase, 1 mM, complete inactivation | Agrobacterium sp. 525a | |
Cu2+ | D-carnitine dehydrogenase, 1 mM, complete inactivation | Agrobacterium sp. 525a | |
Hg2+ | D-carnitine dehydrogenase, 1 mM, complete inactivation | Agrobacterium sp. 525a | |
N-ethylmaleimide | D-carnitine dehydrogenase, 1 mM, 77% inactivation after 3 min | Agrobacterium sp. 525a | |
p-chloromercuribenzoate | D-carnitine dehydrogenase, 0.1 mM, complete inactivation | Agrobacterium sp. 525a |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NADH | D-carnitine dehydrogenase | Agrobacterium sp. 525a | |
0.07 | - |
NAD+ | D-carnitine dehydrogenase | Agrobacterium sp. 525a | |
0.8 | - |
3-dehydrocarnitine | D-carnitine dehydrogenase | Agrobacterium sp. 525a | |
3.1 | - |
D-carnitine | D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | D-carnitine dehydrogenase, 0.5 mM, 17% increase in activity, 1.0 mM 30% increase in activity, 5.0 mM, 35% increase in activity | Agrobacterium sp. 525a |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
3 * 29000, D-carnitine dehydrogenase, SDS-PAGE | Agrobacterium sp. 525a |
86000 | - |
gel filtration, D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | Agrobacterium sp. 525a | - |
3-dehydrocarnitine + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium sp. 525a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl, ammonium sulfate, Sephadex G-75, 5'-AMP-Sepharose 4B, Mono Q, TSK-gel G3000 SW, D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.7 | - |
D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | - |
Agrobacterium sp. 525a | 3-dehydrocarnitine + NADH + H+ | - |
r | |
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | D-carnitine dehydrogenase | Agrobacterium sp. 525a | 3-dehydrocarnitine + NADH + H+ | - |
r | |
3-hydroxy-2-butanone + NAD+ | enzyme specific for D-carnitine | Agrobacterium sp. 525a | 2,3-butandione + NADH | - |
? | |
DL-4-amino-3-hydroxy-n-butyrate + NAD+ | D-carnitine dehydrogenase | Agrobacterium sp. 525a | DL-4-amino-3-keto-n-butyrate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 29000, D-carnitine dehydrogenase, SDS-PAGE | Agrobacterium sp. 525a |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
D-carnitine dehydrogenase, enzyme is rapidly inactivated above 40°C | Agrobacterium sp. 525a |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | D-carnitine dehydrogenase, reduction of 3-dehydrocarnitine | Agrobacterium sp. 525a |
9.3 | - |
D-carnitine dehydrogenase, oxidation of D-carnitine | Agrobacterium sp. 525a |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | D-carnitine dehydrogenase | Agrobacterium sp. 525a |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Agrobacterium sp. 525a |