BRENDA - Enzyme Database
show all sequences of 1.1.1.25

Identification of novel polyphenolic inhibitors of shikimate dehydrogenase (AroE)

Peek, J.; Shi, T.; Christendat, D.; J. Biomol. Screen. 19, 1090-1098 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Arabidopsis thaliana
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Pseudomonas putida
Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystal structure analysis, PDB ID 2O7S
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2-bisepigallocatechin gallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
aurintricarboxylic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
baicalein
about 25% inhibition at 0.0025 mM
Pseudomonas putida
cardiolipin
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
dianthrol
about 50% inhibition at 0.0025 mM
Pseudomonas putida
diethylenetriamine pentaacetic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
ebselen
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
ellagic acid
about 50% inhibition at 0.0025 mM
Pseudomonas putida
epicatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epicatechin gallate
over 75% inhibition at 0.0025 mM
Pseudomonas putida
epigallocatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epigallocatechin gallate
over 75% inhibition at 0.0025 mM
Pseudomonas putida
epigallocatechin-3,5-digallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
epitheaflavin monogallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
hydroquinone
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
merbromin
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
additional information
screening for polyphenolc enzyme inhibitors, overview
Arabidopsis thaliana
additional information
screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin
Pseudomonas putida
nordihydroguaiaretic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
purpurogallin
about 50% inhibition at 0.0025 mM
Pseudomonas putida
pyridoxine
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
pyrogallin
about 50% inhibition at 0.0025 mM
Pseudomonas putida
quercetin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
taxifolin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
theaflavanin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
theaflavin monogallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
theaflavin-3,3-digallate
about 25% inhibition at 0.0025 mM
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-dehydroshikimate + NADPH
Arabidopsis thaliana
-
shikimate + NADP+
-
-
r
shikimate + NADP+
Pseudomonas putida
-
3-dehydroshikimate + NADPH + H+
-
-
r
shikimate + NADP+
Pseudomonas putida KT 2240
-
3-dehydroshikimate + NADPH + H+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q9SQT8
-
-
Pseudomonas putida
Q88RQ5
-
-
Pseudomonas putida KT 2240
Q88RQ5
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Arabidopsis thaliana
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-dehydroshikimate + NADPH
-
740771
Arabidopsis thaliana
shikimate + NADP+
-
-
-
r
additional information
The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate
740771
Arabidopsis thaliana
?
-
-
-
?
shikimate + NADP+
-
740771
Pseudomonas putida
3-dehydroshikimate + NADPH + H+
-
-
-
r
shikimate + NADP+
-
740771
Pseudomonas putida KT 2240
3-dehydroshikimate + NADPH + H+
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
AroE
-
Arabidopsis thaliana
AroE
-
Pseudomonas putida
bifunctional dehydroquinate dehydratase-shikimate dehydrogenase
-
Arabidopsis thaliana
DHQ-SDH
-
Arabidopsis thaliana
SDH
-
Arabidopsis thaliana
SDH
-
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
25
-
assay at
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
assay at
Arabidopsis thaliana
8.8
-
assay at
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADP+
-
Pseudomonas putida
NADPH
-
Arabidopsis thaliana
NADPH
-
Pseudomonas putida
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.002
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epicatechin gallate
0.0021
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epigallocatechin gallate
0.003
-
pH 8.8, 25°C, recombinant enzyme
Pseudomonas putida
epigallocatechin gallate
0.0037
-
pH 8.8, 25°C, recombinant enzyme
Pseudomonas putida
epicatechin gallate
Cloned(Commentary) (protein specific)
Commentary
Organism
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Arabidopsis thaliana
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADP+
-
Pseudomonas putida
NADPH
-
Arabidopsis thaliana
NADPH
-
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure analysis, PDB ID 2O7S
Arabidopsis thaliana
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.002
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epicatechin gallate
0.0021
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme
Arabidopsis thaliana
epigallocatechin gallate
0.003
-
pH 8.8, 25°C, recombinant enzyme
Pseudomonas putida
epigallocatechin gallate
0.0037
-
pH 8.8, 25°C, recombinant enzyme
Pseudomonas putida
epicatechin gallate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2-bisepigallocatechin gallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
aurintricarboxylic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
baicalein
about 25% inhibition at 0.0025 mM
Pseudomonas putida
cardiolipin
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
dianthrol
about 50% inhibition at 0.0025 mM
Pseudomonas putida
diethylenetriamine pentaacetic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
ebselen
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
ellagic acid
about 50% inhibition at 0.0025 mM
Pseudomonas putida
epicatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epicatechin gallate
over 75% inhibition at 0.0025 mM
Pseudomonas putida
epigallocatechin gallate
inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana
Arabidopsis thaliana
epigallocatechin gallate
over 75% inhibition at 0.0025 mM
Pseudomonas putida
epigallocatechin-3,5-digallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
epitheaflavin monogallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
hydroquinone
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
merbromin
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
additional information
screening for polyphenolc enzyme inhibitors, overview
Arabidopsis thaliana
additional information
screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin
Pseudomonas putida
nordihydroguaiaretic acid
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
purpurogallin
about 50% inhibition at 0.0025 mM
Pseudomonas putida
pyridoxine
lower inhibitry potency, about 25% inhibition at 0.0025 mM
Pseudomonas putida
pyrogallin
about 50% inhibition at 0.0025 mM
Pseudomonas putida
quercetin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
taxifolin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
theaflavanin
about 25% inhibition at 0.0025 mM
Pseudomonas putida
theaflavin monogallate
about 50% inhibition at 0.0025 mM
Pseudomonas putida
theaflavin-3,3-digallate
about 25% inhibition at 0.0025 mM
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-dehydroshikimate + NADPH
Arabidopsis thaliana
-
shikimate + NADP+
-
-
r
shikimate + NADP+
Pseudomonas putida
-
3-dehydroshikimate + NADPH + H+
-
-
r
shikimate + NADP+
Pseudomonas putida KT 2240
-
3-dehydroshikimate + NADPH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Arabidopsis thaliana
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-dehydroshikimate + NADPH
-
740771
Arabidopsis thaliana
shikimate + NADP+
-
-
-
r
additional information
The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate
740771
Arabidopsis thaliana
?
-
-
-
?
shikimate + NADP+
-
740771
Pseudomonas putida
3-dehydroshikimate + NADPH + H+
-
-
-
r
shikimate + NADP+
-
740771
Pseudomonas putida KT 2240
3-dehydroshikimate + NADPH + H+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
25
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
assay at
Arabidopsis thaliana
8.8
-
assay at
Pseudomonas putida
Other publictions for EC 1.1.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740821
Bontpart
Two shikimate dehydrogenases, ...
Vitis vinifera
J. Exp. Bot.
67
3537-3550
2016
-
-
4
-
-
-
-
13
1
-
-
4
-
6
-
-
-
-
-
15
-
-
8
-
8
4
-
-
12
7
-
-
8
-
-
-
-
-
4
8
-
-
-
-
-
-
13
1
-
-
4
-
-
-
-
-
15
-
-
8
-
4
-
-
12
7
-
-
-
-
8
8
-
12
12
739956
Peek
The shikimate dehydrogenase fa ...
Escherichia coli, Helicobacter pylori, Staphylococcus aureus, Populus trichocarpa, Mycobacterium tuberculosis, Corynebacterium glutamicum, Archaeoglobus fulgidus, Aquifex aeolicus, Aspergillus nidulans, Staphylococcus epidermidis, Toxoplasma gondii, Pseudomonas putida, Arabidopsis thaliana, Archaeoglobus fulgidus ATCC 49558, Aspergillus nidulans FGSC A4, Staphylococcus epidermidis ATCC 35984, Pseudomonas putida KT 2240
Arch. Biochem. Biophys.
566
85-99
2015
-
11
1
4
6
-
2
30
-
-
-
17
-
19
-
-
-
-
-
-
1
-
27
7
19
12
-
-
26
11
1
-
23
-
-
-
-
11
1
23
4
6
-
-
2
-
30
-
-
-
17
-
-
-
-
-
-
1
-
27
7
12
-
-
26
11
1
-
-
-
38
38
-
-
-
740347
Prus-Glowacki
-
Shikimate dehydrogenase (E.C. ...
Pinus sylvestris
Dendrobiology
73
153-162
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741401
Li
-
SAR studies on 1,2,4-triazolo[ ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
RSC Adv.
5
97089-97101
2015
-
1
-
-
-
-
26
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
26
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
741411
Zhang
Determination of the cytosolic ...
Escherichia coli
Sci. Rep.
5
12846
2015
-
1
1
-
1
-
-
-
3
-
-
1
-
4
-
-
-
-
-
-
2
-
2
-
2
2
-
-
-
2
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
2
-
2
-
2
-
-
-
2
-
-
-
-
1
1
-
-
-
740724
Guo
Molecular characterization of ...
Populus trichocarpa, Populus trichocarpa Nisqually-1
J. Biol. Chem.
289
23846-23858
2014
-
-
2
-
-
-
-
5
1
-
-
7
-
8
-
-
1
-
-
5
-
-
15
2
4
1
-
-
-
1
-
-
6
-
-
-
-
-
2
6
-
-
-
-
-
-
5
1
-
-
7
-
-
-
1
-
5
-
-
15
2
1
-
-
-
1
-
-
-
-
5
5
-
-
-
740771
Peek
Identification of novel polyph ...
Pseudomonas putida, Arabidopsis thaliana, Pseudomonas putida KT 2240
J. Biomol. Screen.
19
1090-1098
2014
-
-
2
1
-
-
27
-
-
-
-
3
-
17
-
-
2
-
-
-
-
-
4
-
6
2
-
-
-
2
-
-
4
-
-
4
-
-
2
4
1
-
-
4
27
-
-
-
-
-
3
-
-
-
2
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
740978
Peek
Isolation and molecular charac ...
Toxoplasma gondii
Mol. Biochem. Parasitol.
194
16-19
2014
-
-
-
-
-
-
2
2
-
-
-
1
-
8
-
-
-
-
-
-
-
-
1
2
1
-
-
-
1
1
-
-
1
-
-
1
-
-
-
1
-
-
-
1
2
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
1
-
-
-
-
3
3
-
-
-
741030
Avitia-Dominguez
Inhibition and biochemical cha ...
Staphylococcus aureus, Staphylococcus aureus ATCC MRSA252
Molecules
19
4491-4509
2014
-
1
1
-
-
-
10
3
-
-
1
2
-
11
-
-
1
1
-
-
-
-
2
2
3
1
1
-
2
1
1
-
1
1
-
-
-
1
1
1
-
-
-
-
10
1
3
-
-
1
2
-
-
-
1
-
-
-
-
2
2
1
1
-
2
1
1
-
-
-
-
-
-
2
2
724089
Kubota
Characterization of shikimate ...
Corynebacterium glutamicum, Corynebacterium glutamicum JCM 18229
Appl. Microbiol. Biotechnol.
97
8139-8149
2013
-
-
2
-
-
-
2
6
-
-
2
-
-
9
-
-
-
-
-
-
-
-
12
2
5
-
-
-
6
4
-
-
6
-
-
-
-
-
2
6
-
-
-
-
4
-
6
-
-
2
-
-
-
-
-
-
-
-
-
12
2
-
-
-
6
4
-
-
-
1
1
1
1
6
6
725977
Lee
High-resolution structure of s ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Mol. Cells
33
229-233
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721203
Lee
Overexpression, crystallizatio ...
Archaeoglobus fulgidus, Archaeoglobus fulgidus ATCC 49558
Acta Crystallogr. Sect. F
67
1556-1558
2011
-
1
2
2
-
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
2
5
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723859
Lee
Overexpression, crystallizatio ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Acta Crystallogr. Sect. F
67
824-826
2011
-
-
1
1
-
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
2
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
4
2
2
-
2
2
-
-
-
1
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
4
2
2
-
2
-
-
-
1
-
-
-
-
1
1
-
-
-
697894
Han
X-ray crystallographic and enz ...
Staphylococcus epidermidis RP62A
FEBS J.
276
1125-1139
2009
-
-
1
1
1
-
-
5
-
-
4
-
-
4
-
-
1
-
-
-
-
-
3
1
1
-
-
-
3
1
1
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
5
-
-
4
-
-
-
-
1
-
-
-
-
3
1
-
-
-
3
1
1
-
-
-
-
-
-
-
-
698320
Rodrigues
Homogeneous recombinant Mycoba ...
Mycobacterium tuberculosis
Int. J. Biol. Macromol.
45
200-205
2009
-
-
1
-
6
-
-
2
1
-
1
-
-
6
-
-
1
-
-
-
2
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
2
1
-
1
-
-
-
-
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
699631
Barcellos
Structural studies of shikimat ...
Bacillus anthracis
J. Mol. Model.
15
147-155
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
711616
Rodrigues
The conserved Lysine69 residue ...
no activity in Homo sapiens, Mycobacterium tuberculosis
BMC Res. Notes
2
227
2009
-
1
-
-
1
-
-
5
-
-
-
1
-
6
-
-
-
1
-
-
-
-
2
1
1
1
-
-
2
1
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
2
1
1
-
-
2
1
-
-
-
-
2
2
-
4
4
684153
Schoepe
1.6 A structure of an NAD(+)-d ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. D
64
803-809
2008
-
-
-
1
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
689956
Arcuri
Structural studies of shikimat ...
Mycobacterium tuberculosis
Proteins
72
720-730
2008
-
-
-
1
-
-
-
-
-
-
-
-
-
16
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
694093
Singh
A phylogenomic analysis of the ...
Pseudomonas putida, Pseudomonas putida KT2440, Pseudomonas putida KT 2240
Mol. Biol. Evol.
25
2221-2232
2008
-
-
7
-
-
-
-
24
-
-
-
12
-
15
-
-
7
-
-
-
-
2
20
-
18
4
-
-
29
4
-
-
7
-
-
-
-
-
7
7
-
-
-
-
-
-
24
-
-
-
12
-
-
-
7
-
-
-
2
20
-
4
-
-
29
4
-
-
-
-
-
-
-
-
-
681134
Ding
Functional analysis of the ess ...
Nicotiana tabacum
J. Exp. Bot.
58
2053-2067
2007
-
-
4
-
4
-
-
4
4
-
-
6
-
4
-
-
2
-
-
2
-
-
11
-
6
4
-
-
-
4
-
-
4
-
-
-
-
-
4
4
-
4
-
-
-
-
4
4
-
-
6
-
-
-
2
-
2
-
-
11
-
4
-
-
-
4
-
-
-
-
-
-
-
-
-
684670
Fonseca
Kinetic and chemical mechanism ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Arch. Biochem. Biophys.
457
123-133
2007
-
-
-
-
-
-
2
2
-
-
-
-
-
8
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
685190
Gan
Structural and biochemical ana ...
Aquifex aeolicus
Biochemistry
46
9513-9522
2007
-
-
1
1
-
-
-
2
-
-
-
-
-
6
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
686243
Singh
-
The DHQ-dehydroshikimate-SDH-s ...
Arabidopsis thaliana
Cryst. Growth Des.
7
2153-2160
2007
-
-
1
1
8
-
-
16
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
688379
Bagautdinov
Crystal structures of shikimat ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
J. Mol. Biol.
373
424-438
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667097
Schoepe
Cloning, expression, purificat ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
F62
635-637
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667700
Singh
Structure of Arabidopsis dehyd ...
Arabidopsis thaliana
Biochemistry
45
10406
2006
-
-
1
1
10
-
-
18
-
-
-
2
-
1
-
-
-
1
-
-
1
-
3
-
4
1
-
-
18
1
-
-
2
-
-
-
-
-
1
2
1
10
-
-
-
-
18
-
-
-
2
-
-
-
-
-
-
1
-
3
-
1
-
-
18
1
-
-
-
-
-
-
-
-
-
668019
Adachi
Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3244
Biosci. Biotechnol. Biochem.
70
2786-2789
2006
-
-
-
-
-
-
-
-
-
-
3
2
-
10
-
-
-
-
-
1
-
-
2
2
2
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
3
2
-
-
-
-
-
1
-
-
2
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
668576
Han
Biochemical characterization a ...
Helicobacter pylori, Helicobacter pylori SS1
FEBS J.
273
4682-4692
2006
-
-
1
-
-
-
6
3
-
-
-
2
-
10
-
-
1
-
-
-
1
-
8
1
1
1
1
-
3
1
1
-
4
-
-
5
-
-
1
4
-
-
-
5
6
-
3
-
-
-
2
-
-
-
1
-
-
1
-
8
1
1
1
-
3
1
1
-
-
-
-
-
-
-
-
670786
Fonseca
Functional shikimate dehydroge ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
46
429-437
2006
-
-
1
-
-
-
-
5
-
-
5
-
-
13
-
-
1
-
-
-
1
-
2
2
2
1
-
2
4
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
5
-
-
-
-
1
-
-
1
-
2
2
1
-
2
4
1
-
-
-
-
-
-
-
-
-
669163
Zhang
Expression, purification and p ...
Mycobacterium tuberculosis
J. Biochem. Mol. Biol.
38
624-631
2005
2
-
1
-
-
1
1
3
-
-
-
1
-
10
-
-
1
-
-
-
1
2
1
1
2
1
-
1
2
1
-
-
2
-
-
-
2
-
1
2
-
-
1
-
1
-
3
-
-
-
1
-
-
-
1
-
-
1
2
1
1
1
-
1
2
1
-
-
-
-
-
-
-
-
-
669328
Singh
Crystal structure of a novel s ...
Haemophilus influenzae
J. Biol. Chem.
280
17101-17108
2005
-
-
1
1
2
-
1
-
-
1
-
2
-
4
-
-
-
1
-
-
-
-
2
1
2
1
-
-
-
2
1
-
2
-
-
-
-
-
1
2
1
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
2
1
-
-
-
-
-
-
-
-
655639
Lim
A thermostable shikimate 5-deh ...
Archaeoglobus fulgidus
FEMS Microbiol. Lett.
238
101-106
2004
-
-
1
-
-
-
-
3
-
-
3
-
-
12
-
-
-
-
-
-
2
-
3
1
-
2
-
6
-
-
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
2
-
3
1
2
-
6
-
-
1
-
-
-
-
-
-
-
-
655854
Ye
The crystal structure of shiki ...
Haemophilus influenzae
J. Bacteriol.
185
4144-4151
2003
-
-
1
2
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657359
Padyana
Crystal structure of shikimate ...
Methanocaldococcus jannaschii
Structure
11
1005-1013
2003
-
-
1
2
-
-
-
-
-
-
2
1
-
11
-
-
1
-
-
-
-
-
1
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
2
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670901
Vogan
Shikimate dehydrogenase struct ...
Methanocaldococcus jannaschii
Structure
11
902-903
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657254
Magalhaes
Cloning and expression of func ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
26
59-64
2002
-
-
1
-
-
-
-
-
-
-
1
-
-
198
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286396
Maclean
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
56
512-515
2000
-
-
2
2
-
-
-
-
-
-
3
1
-
6
-
-
2
-
-
-
-
1
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
2
-
-
-
-
-
-
-
-
3
1
-
-
-
2
-
-
-
1
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286398
Diaz
-
Shikimate dehydrogenase from p ...
Capsicum annuum
Physiol. Plant.
100
147-152
1997
4
-
-
-
-
-
5
2
-
-
1
2
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
5
-
2
-
-
1
2
-
-
-
1
-
-
-
-
2
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-
286397
Lourenco
Purification and properties of ...
Cucumis sativus
J. Agric. Food Chem.
39
458-462
1991
-
-
-
-
-
1
5
2
-
-
1
1
-
1
-
-
1
-
-
-
2
-
1
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
2
-
-
1
1
-
-
-
1
-
-
2
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
286386
Chaudhuri
The purification of shikimate ...
Escherichia coli
Biochem. J.
226
217-223
1985
-
-
-
-
-
-
-
-
-
-
1
1
-
6
-
-
1
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286394
Lemos Silva
-
Inhibition of shikimate dehydr ...
Euterpe oleracea
J. Food Biochem.
9
105-116
1985
-
-
-
-
-
-
7
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286387
Lourenco
-
Partial purification and some ...
Solanum lycopersicum
Phytochemistry
23
497-499
1984
-
-
-
-
-
-
8
2
-
1
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
8
-
2
-
1
1
1
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
5466
Polley
Purification and characterizat ...
Physcomitrium patens
Biochim. Biophys. Acta
526
259-266
1978
-
-
-
-
-
-
-
-
-
-
1
1
-
6
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
286388
Koshiba
Purification of two forms of t ...
Vigna mungo
Biochim. Biophys. Acta
522
10-18
1978
-
-
-
-
-
-
4
3
-
-
1
1
-
4
-
-
1
-
-
2
1
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
1
1
-
-
-
1
-
2
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
5464
Jacobson
Purification and stability of ...
Neurospora crassa
Biochim. Biophys. Acta
289
1-12
1972
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286390
Higuchi
-
Changes in activity of shikima ...
Bambusa sp.
Plant Cell Physiol.
8
61-69
1967
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286389
Sanderson
5-Dehydroshikimate reductase i ...
Camellia sinensis
Biochem. J.
98
248-252
1966
-
-
-
-
-
-
3
2
-
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
286393
Balinsky
Aromatic biosynthesis in highe ...
Pisum sativum
Biochem. J.
80
292-296
1961
-
-
-
-
-
-
2
2
-
1
1
3
-
3
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
1
1
3
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286395
Yaniv
Aromatic biosynthesis. XIV. 5- ...
Escherichia coli
J. Biol. Chem.
213
787-795
1955
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-