Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,5-dichlorosalicylic acid | uncompetitive inhibition | Homo sapiens | |
minalrestat | uncompetitive inhibition | Homo sapiens | |
additional information | Arg312 in ALR1 contributes favourably to the binding of 3,5-dichlorosalicylic acid through an electrostatic interaction with the inhibitor's electronegative halide atom and undergoes a conformational change upon tolrestat binding. Tyr116 in ALR1 forms electrostatic interactions with the fluorobenzyl moiety of minalrestat and the 3- and 4-hydroxy groups on the phenyl ring of quercetin. | Homo sapiens | |
quercetin | uncompetitive inhibition | Homo sapiens | |
Tolrestat | uncompetitive inhibition | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15121 | - |
- |
Purification (Comment) | Organism |
---|---|
Blue Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde + NADPH + H+ | - |
Homo sapiens | glycerol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldose reductase | - |
Homo sapiens |
ALR2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00026 | - |
ALR2 in 50 mM potassium phosphate (pH 6.2) | Homo sapiens | Tolrestat | |
0.00028 | - |
ALR2 in 50 mM potassium phosphate (pH 6.2) | Homo sapiens | minalrestat | |
0.00592 | - |
ALR2 in 50 mM potassium phosphate (pH 6.2) | Homo sapiens | quercetin | |
0.127 | - |
ALR2 in 50 mM potassium phosphate (pH 6.2) | Homo sapiens | 3,5-dichlorosalicylic acid |