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Literature summary for 1.1.1.206 extracted from

  • Hashimoto, T.; Nakajima, K.; Ongena, G.; Yamada, Y.
    Two tropinone reductases with distinct stereospecificities from cultured roots of Hyoscyamus niger (1992), Plant Physiol., 100, 836-845.
    View publication on PubMed
Search for more literature for 1.1.1.206

Inhibitors

Inhibitors Comment Organism Structure
8-thiabicyclo(3,2,1)octane-3-one complete inhibition at 4 mM Hyoscyamus niger
Ca2+ 0-15% inactivation at 1 mM Hyoscyamus niger
Cd2+ 26-43% inactivation at 1 mM Hyoscyamus niger
Co2+ 0-15% inactivation at 1 mM Hyoscyamus niger
Cu2+ 99% inactivation at 1 mM Hyoscyamus niger
diethyl dicarbonate complete inhibition at 0.1 mM Hyoscyamus niger
Fe2+ 0-15% inactivation at 1 mM Hyoscyamus niger
Fe3+ 0-15% inactivation at 1 mM Hyoscyamus niger
Hg2+ 99% inactivation at 1 mM Hyoscyamus niger
Li+ 0-15% inactivation at 1 mM Hyoscyamus niger
Mg2+ 0-15% inactivation at 1 mM Hyoscyamus niger
Mn2+ 0-15% inactivation at 1 mM Hyoscyamus niger
additional information not inhibited by EDTA and iodoacetamide Hyoscyamus niger
Ni2+ 0-15% inactivation at 1 mM Hyoscyamus niger
p-chloromercuribenzoate strong inhibition at 0.1 and 1.0 mM Hyoscyamus niger
Zn2+ 0-15% inactivation at 1 mM Hyoscyamus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 4-methylcyclohexanone
-
 Hyoscyamus niger
0.003
-
 4-ethylcyclohexanone
-
 Hyoscyamus niger
0.003
-
 8-thiabicyclo(3,2,1)octane-3-one
-
 Hyoscyamus niger
0.004
-
 3-methylcyclohexanone
-
 Hyoscyamus niger
0.011
-
 NADPH
-
 Hyoscyamus niger
0.042
-
 NADP+
-
 Hyoscyamus niger
0.231
-
 N-Methyl-4-piperidone
-
 Hyoscyamus niger
1.01
-
 tropinone
-
 Hyoscyamus niger
1.81
-
 3-quinuclidinone
-
 Hyoscyamus niger
2.6
-
 Tropine
-
 Hyoscyamus niger
10
-
 NADH
-
 Hyoscyamus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Hyoscyamus niger
115000
-
 gel filtration Hyoscyamus niger

Organism

Organism UniProt Comment Textmining
Atropa acuminata
-
-
-
Atropa belladonna
-
-
-
Datura stramonium
-
-
-
Duboisia hopwoodii
-
-
-
Duboisia leichhardtii
-
-
-
Hyoscyamus albus
-
-
-
Hyoscyamus bohemicus
-
-
-
Hyoscyamus canariensis
-
-
-
Hyoscyamus muticus
-
-
-
Hyoscyamus niger
-
 and strain Hn11
-
Hyoscyamus niger Hn
-
 and strain Hn11
-
Hyoscyamus pusillus
-
-
-
no activity in Brassica campestris
-
-
-
no activity in Browallia americana
-
-
-
no activity in Calystegia sepium
-
-
-
no activity in Nicotiana tabacum
-
-
-
no activity in Physalis alkekengi
-
-
-
Physalis edulis
-
-
-
Physalis philadelphica
-
-
-
Physochlaina orientalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Datura stramonium
-
root
-
 Datura stramonium
-
root
-
 Hyoscyamus niger
-
root
-
 Atropa belladonna
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 tropinone Hyoscyamus albus
1.2
-
 tropinone Hyoscyamus bohemicus
1.2
-
 tropinone Atropa acuminata
3
-
 tropinone Physochlaina orientalis
7.5
-
 tropinone, strain Hn Hyoscyamus niger
7.5
-
 tropinone Hyoscyamus canariensis
7.5
-
 tropinone Atropa belladonna
10.2
-
 tropinone Hyoscyamus pusillus
10.8
-
 tropinone Hyoscyamus muticus
15
-
 tropinone, strain Hn11 Hyoscyamus niger
30
-
 tropinone Duboisia leichhardtii
30
-
 tropinone Duboisia hopwoodii
45
-
 tropinone Datura stramonium
54
-
 tropinone Physalis edulis
66
-
 tropinone Physalis philadelphica

Storage Stability

Storage Stability Organism
4°C, NADPH, NADP+ and glycerol enhance stability Hyoscyamus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcyclohexanone + NADPH
-
 Datura stramonium 3-methylcyclohexanol + NADP+
-
 ?
3-methylcyclohexanone + NADPH
-
 Hyoscyamus niger 3-methylcyclohexanol + NADP+
-
 ?
3-methylcyclohexanone + NADPH
-
 Hyoscyamus niger Hn 3-methylcyclohexanol + NADP+
-
 ?
3-quinuclidinone + NADPH + H+
-
 Datura stramonium quinuclidin-3-ol + NADP+
-
 ?
3-quinuclidinone + NADPH + H+
-
 Hyoscyamus niger quinuclidin-3-ol + NADP+
-
 ?
4-ethylcyclohexanone + NADPH
-
 Datura stramonium 4-ethylcyclohexanol + NADP+
-
 ?
4-ethylcyclohexanone + NADPH
-
 Hyoscyamus niger 4-ethylcyclohexanol + NADP+
-
 ?
4-ethylcyclohexanone + NADPH
-
 Hyoscyamus niger Hn 4-ethylcyclohexanol + NADP+
-
 ?
4-methylcyclohexanone + NADPH
-
 Datura stramonium 4-methylcyclohexanol + NADP+
-
 ?
4-methylcyclohexanone + NADPH
-
 Hyoscyamus niger 4-methylcyclohexanol + NADP+
-
 ?
4-methylcyclohexanone + NADPH
-
 Hyoscyamus niger Hn 4-methylcyclohexanol + NADP+
-
 ?
8-thiabicyclo(3,2,1)octane-3-one + NADPH
-
 Hyoscyamus niger 8-thiabicyclo(3,2,1)octane-3-ol + NADP+
-
 ?
N-methyl-4-piperidone + NADPH
-
 Datura stramonium 1-methylpiperidin-4-ol + NADP+
-
 ?
N-methyl-4-piperidone + NADPH
-
 Hyoscyamus niger 1-methylpiperidin-4-ol + NADP+
-
 ?
N-methyl-4-piperidone + NADPH
-
 Hyoscyamus niger Hn 1-methylpiperidin-4-ol + NADP+
-
 ?
tropine + NADP+
-
 Datura stramonium tropinone + NADPH
-
 r
tropine + NADP+
-
 Hyoscyamus niger tropinone + NADPH
-
 r
tropine + NADP+
-
 Hyoscyamus niger Hn tropinone + NADPH
-
 r
tropinone + NADH + H+
-
 Hyoscyamus niger tropine + NAD+
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 30000, gel filtration Hyoscyamus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
 tropinone reduction, narrow pH-range Hyoscyamus niger
7.6
-
 tropine oxidation Hyoscyamus niger

Cofactor

Cofactor Comment Organism Structure
NADH lower affinity than with NADPH Hyoscyamus niger
NADPH
-
 Datura stramonium
NADPH
-
 Hyoscyamus niger