Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2
Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.; Microbiology 155, 2078-2085 (2009) 
Data extracted from this reference:
Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli; expression in Escherichia coli
Geobacillus thermodenitrificans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
Co2+
1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
Cu2+
1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
K+
1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
Mg2+
1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
Mn2+
1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
Ni2+
1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
sodium dodecylsulfate
1 mM, 3% residual activity; 1 mM, no residual activity
Geobacillus thermodenitrificans
Zn2+
1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
1 mM, 136% of initial activity; 1 mM, 168% of initial activity
Geobacillus thermodenitrificans
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases; enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
90000
-
PAGE
Geobacillus thermodenitrificans
380000
-
PAGE
Geobacillus thermodenitrificans
Organism
Organism
UniProt
Commentary
Textmining
Geobacillus thermodenitrificans
A4IP64
-
-
Geobacillus thermodenitrificans
A4ISB9
-
-
Geobacillus thermodenitrificans NG80-2
A4IP64
-
-
Geobacillus thermodenitrificans NG80-2
A4ISB9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1-decanol + 2 NAD+ + H2O
about 20% of the activity with ethanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1-decanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1-dodecanol + 2 NAD+ + H2O
about 17% of the activity with ethanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-dodecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-eicosanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-eicosanol + 2 NAD+ + H2O
about 3% of the activity with ethanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-hexadecanol + 2 NAD+ + H2O
about 10% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-hexadecanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octacosanol + 2 NAD+ + H2O
about 40% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octacosanol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octadecanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octadecanol + 2 NAD+ + H2O
about 5% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octanol + 2 NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetradecanol + 2 NAD+ + H2O
about 15% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetradecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
decanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + NADH
-
-
-
r
dodecanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + NADH
-
-
-
r
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
octanal + NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
Synonyms
Synonyms
Commentary
Organism
ADH1
-
Geobacillus thermodenitrificans
ADH2
-
Geobacillus thermodenitrificans
long-chain alkyl alcohol dehydrogenase
-
Geobacillus thermodenitrificans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
;
Geobacillus thermodenitrificans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
14 h, more than 50% of initial activity; 14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
;
Geobacillus thermodenitrificans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
;
Geobacillus thermodenitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
NADH
-
Geobacillus thermodenitrificans
NADP+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
NADPH
-
Geobacillus thermodenitrificans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Geobacillus thermodenitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
NAD+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
NADH
-
Geobacillus thermodenitrificans
NADP+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
NADP+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
NADPH
-
Geobacillus thermodenitrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
Al3+
1 mM, 14.7% residual activity
Geobacillus thermodenitrificans
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
Co2+
1 mM, 51.9% residual activity
Geobacillus thermodenitrificans
Co2+
1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
Cu2+
1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
Cu2+
1 mM, 14.0% residual activity
Geobacillus thermodenitrificans
K+
1 mM, 70.3% residual activity
Geobacillus thermodenitrificans
K+
1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
Mg2+
1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
Mg2+
1 mM, 0.3% residual activity
Geobacillus thermodenitrificans
Mn2+
1 mM, 14.3% residual activity
Geobacillus thermodenitrificans
Mn2+
1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
Ni2+
1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
Ni2+
1 mM, 18.2% residual activity
Geobacillus thermodenitrificans
sodium dodecylsulfate
1 mM, 3% residual activity
Geobacillus thermodenitrificans
sodium dodecylsulfate
1 mM, no residual activity
Geobacillus thermodenitrificans
Zn2+
1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
Zn2+
1 mM, 14.1% residual activity
Geobacillus thermodenitrificans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
1 mM, 136% of initial activity
Geobacillus thermodenitrificans
Fe2+
1 mM, 168% of initial activity
Geobacillus thermodenitrificans
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
90000
-
PAGE
Geobacillus thermodenitrificans
380000
-
PAGE
Geobacillus thermodenitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1-decanol + 2 NAD+ + H2O
about 20% of the activity with ethanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1-decanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1-dodecanol + 2 NAD+ + H2O
about 17% of the activity with ethanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-dodecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-eicosanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-eicosanol + 2 NAD+ + H2O
about 3% of the activity with ethanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-hexadecanol + 2 NAD+ + H2O
about 10% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-hexadecanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octacosanol + 2 NAD+ + H2O
about 40% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octacosanol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octadecanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octadecanol + 2 NAD+ + H2O
about 5% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octanol + 2 NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetradecanol + 2 NAD+ + H2O
about 15% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetradecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
decanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + NADH
-
-
-
r
dodecanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + NADH
-
-
-
r
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
octanal + NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Geobacillus thermodenitrificans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Geobacillus thermodenitrificans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Geobacillus thermodenitrificans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712962
Liu
Two novel metal-independent lo ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
155
2078-2085
2009
1
-
1
-
-
-
10
5
-
3
4
-
-
4
-
-
-
-
-
-
-
-
26
1
4
1
-
1
3
1
1
-
4
-
-
-
1
-
2
6
-
-
-
-
19
-
5
-
5
4
-
-
-
-
-
-
-
-
-
26
1
2
-
2
3
2
2
-
-
-
-
-
-
5
5
656603
Luesch
Biosynthesis of 4-methylprolin ...
Nostoc sp.
J. Org. Chem.
68
83-91
2003
-
-
1
-
-
-
-
2
-
1
1
1
-
4
-
-
1
-
-
-
-
-
4
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347799
Nagashima
-
Long-chain n-alkanol dehydroge ...
Pseudomonas putida
J. Ferment. Bioeng.
82
328-333
1996
-
-
-
-
-
-
-
11
-
1
2
-
-
1
-
-
1
-
-
-
-
-
9
2
-
1
-
2
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
1
2
-
-
-
-
1
-
-
-
-
9
2
1
-
2
-
2
-
-
-
-
-
-
-
-
-
288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
-
-
-
-
-
-
5
7
-
1
2
1
-
7
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
7
-
1
2
1
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347798
Ribas de Pouplana
-
Structural properties of long- ...
Equus caballus
Biochem. J.
276
433-438
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347795
Ueda
Long-chain alcohol dehydrogena ...
Candida tropicalis, Yarrowia lipolytica
Methods Enzymol.
188
171-175
1990
-
-
-
-
-
6
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
9
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347796
Yamada
Subcellular localization of lo ...
Candida tropicalis
Arch. Microbiol.
128
145-151
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347794
Lee
Characterization of fatty alco ...
Rattus norvegicus
J. Biol. Chem.
254
2892-2896
1979
1
-
-
-
-
-
3
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347800
Eklund
Three-dimensional structure of ...
Equus caballus
J. Mol. Biol.
102
27-59
1976
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-