Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: up to 1.0 mM Zn2+, Mg2+, Mn2+, Fe2+, or EDTA | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0612 | - |
NADPH | pH 7.0, 25°C | Gluconobacter oxydans | |
0.075 | - |
NADPH | pH 7.0, 25°C | Gluconobacter oxydans | |
2.2 | - |
methylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans | |
4.8 | - |
Phenylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans | |
5.7 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans | |
10.8 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31600 | - |
2 * 31600, SDS-PAGE | Gluconobacter oxydans |
66100 | - |
PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FQJ0 | - |
- |
Gluconobacter oxydans | Q5FT75 | - |
- |
Gluconobacter oxydans 621H | Q5FQJ0 | - |
- |
Gluconobacter oxydans 621H | Q5FT75 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
substrate ethyl pyruvate, pH 7.0, 25°C | Gluconobacter oxydans |
3.4 | - |
substrate methylglyoxal, pH 7.0, 25°C | Gluconobacter oxydans |
4.7 | - |
substrate isoamyl pyruvate, pH 7.0, 25°C | Gluconobacter oxydans |
5.4 | - |
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C | Gluconobacter oxydans |
6 | - |
substrate 2,3-butanedione, pH 7.0, 25°C | Gluconobacter oxydans |
6.5 | - |
substrate 2,3-hexanedione, pH 7.0, 25°C | Gluconobacter oxydans |
17 | - |
substrate 2,3-pentanedione, pH 7.0, 25°C | Gluconobacter oxydans |
19.1 | - |
substrate phenylglyoxal, pH 7.0, 25°C | Gluconobacter oxydans |
35 | - |
substrate methylglyoxal, pH 7.0, 25°C | Gluconobacter oxydans |
50.1 | - |
substrate 2,3-hexanedione, pH 7.0, 25°C | Gluconobacter oxydans |
60 | - |
substrate 2,3-butanedione, pH 7.0, 25°C | Gluconobacter oxydans |
63.1 | - |
substrate isoamyl pyruvate, pH 7.0, 25°C | Gluconobacter oxydans |
65 | - |
substrate 2,5-diketo-D-gluconate, pH 7.0, 25°C | Gluconobacter oxydans |
69.6 | - |
substrate 2,3-pentanedione, pH 7.0, 25°C | Gluconobacter oxydans |
74.7 | - |
substrate phenylglyoxal, pH 7.0, 25°C | Gluconobacter oxydans |
75.4 | - |
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C | Gluconobacter oxydans |
92.8 | - |
substrate ethyl pyruvate, pH 7.0, 25°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans | (R)-1-phenyl-2-hydroxy-1-propanone + NADP+ | - |
? | |
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans 621H | (R)-1-phenyl-2-hydroxy-1-propanone + NADP+ | - |
? | |
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans | (S)-1-phenyl-2-hydroxy-1-propanone + NADP+ | - |
? | |
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans 621H | (S)-1-phenyl-2-hydroxy-1-propanone + NADP+ | - |
? | |
2,3-butanedione + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? | |
2,3-butanedione + NADPH + H+ | - |
Gluconobacter oxydans 621H | ? + NADP+ | - |
? | |
2,3-hexanedione + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? | |
2,3-hexanedione + NADPH + H+ | - |
Gluconobacter oxydans 621H | ? + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Gluconobacter oxydans | (R)-2-hydroxy-pentane-3-one + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Gluconobacter oxydans 621H | (R)-2-hydroxy-pentane-3-one + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Gluconobacter oxydans | (S)-2-hydroxy-pentane-3-one + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Gluconobacter oxydans 621H | (S)-2-hydroxy-pentane-3-one + NADP+ | - |
? | |
2,5-diketo-D-gluconate + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? | |
ethyl pyruvate + NADPH + H+ | - |
Gluconobacter oxydans | ethyl lactate + NADP+ | - |
? | |
isoamyl pyruvate + NADPH + H+ | - |
Gluconobacter oxydans | isoamyl lactate + NADP+ | - |
? | |
methylglyoxal + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? | |
additional information | reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone | Gluconobacter oxydans | ? | - |
? | |
additional information | reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone | Gluconobacter oxydans | ? | - |
? | |
additional information | reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone | Gluconobacter oxydans 621H | ? | - |
? | |
additional information | reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone | Gluconobacter oxydans 621H | ? | - |
? | |
phenylglyoxal + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31600, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
Gox0644 | - |
Gluconobacter oxydans |
Gox1615 | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans | |
23.8 | - |
methylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans | |
36.7 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans | |
39.3 | - |
Phenylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Gluconobacter oxydans |
7 | - |
- |
Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.99 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans | |
6.4 | - |
2,3-Pentanedione | pH 7.0, 25°C | Gluconobacter oxydans | |
8.2 | - |
Phenylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans | |
11 | - |
methylglyoxal | pH 7.0, 25°C | Gluconobacter oxydans |