KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
NAD+ | - |
Bacillus subtilis | |
18 | - |
myo-inositol | - |
Bacillus subtilis | |
56 | - |
alpha-D-glucose | - |
Bacillus subtilis | |
167 | - |
D-glucose | - |
Bacillus subtilis | |
190 | - |
D-xylose | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Bacillus subtilis |
160000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.3 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucopyranose + NAD+ | 4fold lower activity compared to myo-inositol as substrate, does not act with the beta-anomer | Bacillus subtilis | D-gluconate + NADH | - |
? | |
D-glucose + NAD+ | - |
Bacillus subtilis | D-gluconate + NADH | - |
? | |
D-xylose + NAD+ | very low activity | Bacillus subtilis | ? + NADH | - |
? | |
myo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimal pH-value for stability | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis |