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Literature summary for 1.1.1.18 extracted from

  • Ramaley, R.; Fujita, Y.; Freese, E.
    Purification and properties of Bacillus subtilis inositol dehydrogenase (1979), J. Biol. Chem., 254, 7684-7690.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
NAD+
-
Bacillus subtilis
18
-
myo-inositol
-
Bacillus subtilis
56
-
alpha-D-glucose
-
Bacillus subtilis
167
-
D-glucose
-
Bacillus subtilis
190
-
D-xylose
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE Bacillus subtilis
160000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + NAD+ Bacillus subtilis
-
2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.3
-
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucopyranose + NAD+ 4fold lower activity compared to myo-inositol as substrate, does not act with the beta-anomer Bacillus subtilis D-gluconate + NADH
-
?
D-glucose + NAD+
-
Bacillus subtilis D-gluconate + NADH
-
?
D-xylose + NAD+ very low activity Bacillus subtilis ? + NADH
-
?
myo-inositol + NAD+
-
Bacillus subtilis 2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
optimal pH-value for stability Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis