General Stability | Organism |
---|---|
thermolabile, stabilizing: substrates or high ionic strength | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | - |
Pseudomonas sp. | |
0.5 | - |
D-xylose | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | Pseudomonas sp. | highly specific for both substrates | D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | Pseudomonas sp. MSU-1 | highly specific for both substrates | D-xylonolactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. MSU-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NAD+-affinity chromatography | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:D-xylose-grown cell | inducible enzyme synthesis by growth in D-xylose-containing medium | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.3 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
frozen, addition of DTT, several weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | highly specific for both substrates | Pseudomonas sp. | D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | highly specific for both substrates | Pseudomonas sp. MSU-1 | D-xylonolactone + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
half-life: 2 min | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cannot be replaced by NADP+ | Pseudomonas sp. |