Cloned (Comment) | Organism |
---|---|
gene lad1, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Trichoderma reesei |
gene ladB, orf JGI183900, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression of gene ladB under the lac promoter, functional co-expression of Aspergillus niger wild-type and C-terminally His6-tagged LadB and LadA, encoding L-arabitol dehydrogenae, in Saccharomyces cerevisiae strain CEN.PK2-1D | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of ladBDELTA enzyme-deficient Aspergillus niger strains | Aspergillus niger |
additional information | construction of ladBDELTA enzyme-deficient Aspergillus niger strains | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-arabitol | pH and temperature not specified in the publication | Trichoderma reesei | |
55 | - |
L-arabitol | pH and temperature not specified in the publication | Aspergillus niger | |
200 | - |
galactitol | about, pH and temperature not specified in the publication | Aspergillus niger | |
200 | - |
galactitol | about, pH and temperature not specified in the publication | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactitol + NAD+ | Aspergillus niger | - |
L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | Trichoderma reesei | L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei | L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | Aspergillus niger ATCC 1015, CBS 113.46 | - |
L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | Trichoderma reesei QM6a | L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei | L-xylo-3-hexulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | Trichoderma reesei | Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase | L-xylulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | Trichoderma reesei QM6a | Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase | L-xylulose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
gene ladB | - |
Aspergillus niger ATCC 1015, CBS 113.46 | - |
gene ladB | - |
Trichoderma reesei | - |
gene lad1 | - |
Trichoderma reesei QM6a | - |
gene lad1 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged LadB from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactitol + NAD+ | - |
Aspergillus niger | L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | - |
Aspergillus niger | L-xylo-3-hexulose + NADH + H+ | HPLC and NMR product analyses | r | |
galactitol + NAD+ | - |
Trichoderma reesei | L-xylo-3-hexulose + NADH + H+ | HPLC and NMR product analyses | r | |
galactitol + NAD+ | L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei | Trichoderma reesei | L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | - |
Aspergillus niger ATCC 1015, CBS 113.46 | L-xylo-3-hexulose + NADH + H+ | - |
r | |
galactitol + NAD+ | - |
Aspergillus niger ATCC 1015, CBS 113.46 | L-xylo-3-hexulose + NADH + H+ | HPLC and NMR product analyses | r | |
galactitol + NAD+ | - |
Trichoderma reesei QM6a | L-xylo-3-hexulose + NADH + H+ | HPLC and NMR product analyses | r | |
galactitol + NAD+ | L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei | Trichoderma reesei QM6a | L-xylo-3-hexulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | - |
Trichoderma reesei | L-xylulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase | Trichoderma reesei | L-xylulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | - |
Trichoderma reesei QM6a | L-xylulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase | Trichoderma reesei QM6a | L-xylulose + NADH + H+ | - |
r | |
L-arabitol + NAD+ | - |
Aspergillus niger | ? + NADH + H+ | - |
r | |
L-arabitol + NAD+ | - |
Aspergillus niger ATCC 1015, CBS 113.46 | ? + NADH + H+ | - |
r | |
additional information | the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol | Aspergillus niger | ? | - |
? | |
additional information | the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol | Trichoderma reesei | ? | - |
? | |
additional information | the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol | Aspergillus niger ATCC 1015, CBS 113.46 | ? | - |
? | |
additional information | the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol | Trichoderma reesei QM6a | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
galactitol dehydrogenase | - |
Aspergillus niger |
Lad1 | - |
Trichoderma reesei |
LadB | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Aspergillus niger | |
NAD+ | dependent on | Trichoderma reesei | |
NADH | dependent on | Aspergillus niger | |
NADH | dependent on | Trichoderma reesei |
Organism | Comment | Expression |
---|---|---|
Trichoderma reesei | gene lad1 is induced in response to L-arabinose, D-galactose, and galactitol | up |
Aspergillus niger | gene ladB is induced in response to D-galactose and galactitol | up |
General Information | Comment | Organism |
---|---|---|
evolution | LadB belongs to the medium-chain dehydrogenases | Aspergillus niger |
malfunction | deletion of ladB in Aspergillus niger results in growth arrest on galactitol and significantly slower growth on D-galactose supplemented with a small amount of D-xylose. D-Galactose alone cannot be utilised by Aspergillus niger and the addition of D-xylose stimulates growth on D-galactose via transcriptional activation of the D-xylose-inducible reductase gene, xyrA | Aspergillus niger |
metabolism | LadB catalyzes a step in the oxido-reductive pathway for D-galactose catabolism in filamentous fungi, overview | Aspergillus niger |