Cloned (Comment) | Organism |
---|---|
subtractive cDNA library screening, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Musca domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0235 | - |
sepiapterin | pH 6.4, 37°C, recombinant enzyme | Musca domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
- |
Musca domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Musca domestica | A0A172EJV6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Musca domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall | larval | Musca domestica | - |
fat body | larval | Musca domestica | - |
larva | - |
Musca domestica | - |
malpighian tubule | larval | Musca domestica | - |
midgut | larval, high enzyme level | Musca domestica | - |
additional information | during the development of Musca domestica, the expression of MDSPR in the larvae is higher than other developmental stages | Musca domestica | - |
trachea | larval | Musca domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sepiapterin + NADPH + H+ | - |
Musca domestica | tetrahydrobiopterin + NADP+ | no stereochemic specification in the publication | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, soluble recombinant His-tagged enzyme, SDS-PAGE | Musca domestica |
Synonyms | Comment | Organism |
---|---|---|
MDSPR | - |
Musca domestica |
sepiapterin reductase | - |
Musca domestica |
SPR | - |
Musca domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Musca domestica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | activity range, profile overview, recombinant His-tagged enzyme, over 50% of maximal activity at 30-85°C | Musca domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme | Musca domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 12 | activity range, profile overview, recombinant His-tagged enzyme, over 50% of maximal activity at pH 3.0-11.0 | Musca domestica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Musca domestica |
Organism | Comment | Expression |
---|---|---|
Musca domestica | infection with Escherichia coli induces the enzyme in Musca domestica larvae, highest after 24 h | up |
General Information | Comment | Organism |
---|---|---|
metabolism | sepiapterin reductase (SPR) catalyzes the final steps of tetrahydrobiopterin (BH4) biosynthesis | Musca domestica |
physiological function | sepiapterin reductase (SPR) catalyzes the final steps of tetrahydrobiopterin (BH4) biosynthesis. BH4 is an essential cofactor for aromatic acid hydroxylases and nitric oxide synthase. BH4 deficiency causes endothelial dysfunction, such as hypertension, atherosclerosis, diabetes, etc. | Musca domestica |