Literature summary for 1.1.1.122 extracted from

Seydametova, E.; Shin, J.; Yu, S.H.; Kim, C.; Kim, H.; Park, Y.J.; Yang, J.K.; Cho, S.; Kim, H.R.; Moon, S.O.; Ban, C.; Kweon, D.H.
Development of a quantitative assay for 2'-fucosyllactose via one-pot reaction with alpha1,2-fucosidase and L-fucose dehydrogenase (2019), Anal. Biochem., 582, 113358 .

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Application

Application	Comment	Organism
biotechnology	the purified alpha1,2-fucosidase and L-fucose dehydrogenase have sufficiently high activities in phosphate-buffered saline (pH 7.0) at 37 °C, making it possible to develop a one-pot method for the quantitative determination of 2'-fucosyllactose in fermentation samples. The application of this method is more convenient for quantifying 2'-fucosyllactose in a variety of samples that may be obtained from different phases of the biotechnological production of this oligosaccharide. The method is useful for simple and rapid screening of active variants during the development of any industrially important microbial strain producing 2'-fucosyllactose	Pseudomonas sp.
diagnostics	the purified alpha1,2-fucosidase and L-fucose dehydrogenase have sufficiently high activities in phosphate-buffered saline (pH 7.0) at 37 °C, making it possible to develop a one-pot method for the quantitative determination of 2'-fucosyllactose in fermentation samples. The application of this method is more convenient for quantifying 2'-fucosyllactose in a variety of samples that may be obtained from different phases of the biotechnological production of this oligosaccharide. The method is useful for simple and rapid screening of active variants during the development of any industrially important microbial strain producing 2'-fucosyllactose	Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli as 6×His-tagged protein	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	SDS-PAGE	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	Q52472	-	-
Pseudomonas sp. 1143	Q52472	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity chromatography	Pseudomonas sp.

Subunits

Subunits	Comment	Organism
monomer	1 * 63000, SDS-PAGE	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
L-fucose dehydrogenase	-	Pseudomonas sp.

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Release 2023.1 (January 2023)