BRENDA - Enzyme Database
show all sequences of 1.1.1.120

L-arabinose/D-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of L-arabinose to L-arabonate with Saccharomyces cerevisiae

Aro-Kaerkkaeinen, N.; Toivari, M.; Maaheimo, H.; Ylilauri, M.; Pentikaeinen, O.T.; Andberg, M.; Oja, M.; Penttilae, M.; Wiebe, M.G.; Ruohonen, L.; Koivula, A.; Appl. Microbiol. Biotechnol. 98, 9653-9665 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain H3756
Rhizobium leguminosarum bv. trifolii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.48
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
0.5
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhizobium leguminosarum bv. trifolii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fucose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
D-fucose + NADP+
100% activity
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
D-galactose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
D-galactono-1,5-lactone + NADH + H+
-
-
-
?
D-galactose + NADP+
69% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
D-galactono-1,5-lactone + NADPH + H+
-
-
-
?
L-arabinose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
L-arabino-1,5-lactone + NADH + H+
-
-
-
?
L-arabinose + NADP+
12% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
L-arabinose + NADP+
21% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
L-arabino-1,5-lactone + NADPH + H+
-
-
-
?
additional information
some minor activity (about 1%) can be measured with D-xylose and L-lyxose, while no activity is detected with D-glucose or L-rhamnose
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 000, SDS-PAGE
Rhizobium leguminosarum bv. trifolii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
38.12
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
69.9
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
Rhizobium leguminosarum bv. trifolii
NADP+
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
Rhizobium leguminosarum bv. trifolii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain H3756
Rhizobium leguminosarum bv. trifolii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
Rhizobium leguminosarum bv. trifolii
NADP+
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
Rhizobium leguminosarum bv. trifolii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.48
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
0.5
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fucose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
D-fucose + NADP+
100% activity
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
D-galactose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
D-galactono-1,5-lactone + NADH + H+
-
-
-
?
D-galactose + NADP+
69% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
D-galactono-1,5-lactone + NADPH + H+
-
-
-
?
L-arabinose + NAD+
-
739943
Rhizobium leguminosarum bv. trifolii
L-arabino-1,5-lactone + NADH + H+
-
-
-
?
L-arabinose + NADP+
12% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
?
L-arabinose + NADP+
21% activity compared to D-fucose
739943
Rhizobium leguminosarum bv. trifolii
L-arabino-1,5-lactone + NADPH + H+
-
-
-
?
additional information
some minor activity (about 1%) can be measured with D-xylose and L-lyxose, while no activity is detected with D-glucose or L-rhamnose
739943
Rhizobium leguminosarum bv. trifolii
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 000, SDS-PAGE
Rhizobium leguminosarum bv. trifolii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
38.12
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
69.9
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
80.17
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
140
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
80.17
-
D-galactose
with NAD+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
140
-
D-galactose
with NADP+ as cosubstrate, at pH 9.0 and 23°C
Rhizobium leguminosarum bv. trifolii
Other publictions for EC 1.1.1.120
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739943
Aro-Kaerkkaeinen
L-arabinose/D-galactose 1-dehy ...
Rhizobium leguminosarum bv. trifolii
Appl. Microbiol. Biotechnol.
98
9653-9665
2014
-
-
1
-
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2
-
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6
-
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8
1
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2
-
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2
-
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1
2
-
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-
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2
-
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8
1
-
-
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2
-
-
-
-
-
-
-
-
2
2
285792
Schiwara
Anreicherung und Charakterisie ...
Pseudomonas putida
Hoppe-Seyler's Z. Physiol. Chem.
349
1321-1329
1968
-
-
-
-
-
1
5
7
-
-
-
1
-
1
-
-
1
-
-
1
1
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
5
-
7
-
-
-
1
-
-
-
1
-
1
1
-
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp.
J. Biol. Chem.
240
4488-4492
1965
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
1
1
1
4
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp.
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
-
-
-
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3
-
1
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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3
-
-
-
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-
1
-
-
14
-
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-
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