Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 105.7% of initial activity | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, 18.9% of initial activity | Geobacillus thermodenitrificans | |
Ca2+ | 1 mM, 98.4% of initial activity | Geobacillus thermodenitrificans | |
Co2+ | 1 mM, 51.9% of initial activity | Geobacillus thermodenitrificans | |
Cu2+ | 1 mM, 44.2% of initial activity | Geobacillus thermodenitrificans | |
K+ | 1 mM, 70.3% of initial activity | Geobacillus thermodenitrificans | |
Mg2+ | 1 mM, 74.35% of initial activity | Geobacillus thermodenitrificans | |
Mn2+ | 1 mM, 14.3% of initial activity | Geobacillus thermodenitrificans | |
Ni2+ | 1 mM, 72.7% of initial activity | Geobacillus thermodenitrificans | |
SDS | 1 mM, 2.9% of initial activity | Geobacillus thermodenitrificans | |
Zn2+ | 1 mM, 30.5% of initial activity | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
NADP+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
1.4 | - |
NADH | cosubstrate acetaldehyde, pH 8, 60°C | Geobacillus thermodenitrificans | |
1.51 | - |
NAD+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
4.71 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
7.55 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM, 136.1% of initial activity | Geobacillus thermodenitrificans | |
additional information | purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn | Geobacillus thermodenitrificans | |
Na+ | 1 mM, 110.5% of initial activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
380000 | - |
PAGE | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4IP64 | cf. EC 1.1.1.6 and EC 1.1.1.192 | - |
Geobacillus thermodenitrificans NG80-2 | A4IP64 | cf. EC 1.1.1.6 and EC 1.1.1.192 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, purification by nickel ion affinity chromatography | Geobacillus thermodenitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
447.6 | - |
substrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + 2 NAD+ + H2O | - |
Geobacillus thermodenitrificans | propanedial + 2 NADH + 2 H+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Geobacillus thermodenitrificans | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Geobacillus thermodenitrificans NG80-2 | ethanol + NAD+ | - |
r | |
butan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | butanal + NADH + H+ | - |
r | |
decan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | decanal + NADH + H+ | - |
r | |
dodecan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | dodecanal + NADH + H+ | - |
r | |
eicosan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | n-eicosanal + NADH + H+ | - |
r | |
ethanol + NAD+ | ethanol is the best substrate | Geobacillus thermodenitrificans | acetaldehyde + NADH + H+ | - |
r | |
ethanol + NADP+ | - |
Geobacillus thermodenitrificans | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Geobacillus thermodenitrificans NG80-2 | acetaldehyde + NADPH + H+ | - |
r | |
formaldehyde + NADH + H+ | - |
Geobacillus thermodenitrificans | methanol + NAD+ | - |
r | |
formaldehyde + NADH + H+ | - |
Geobacillus thermodenitrificans NG80-2 | methanol + NAD+ | - |
r | |
glycerol + NAD+ | - |
Geobacillus thermodenitrificans | dihydroxyacetone + NADH + H+ | - |
r | |
glycerol + NAD+ | - |
Geobacillus thermodenitrificans NG80-2 | dihydroxyacetone + NADH + H+ | - |
r | |
hexadecan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | hexadecanal + NADH + H+ | - |
r | |
hexan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | hexanal + NADH + H+ | - |
r | |
isoamylalcohol + NAD+ | - |
Geobacillus thermodenitrificans | 3-methylbutanal + NADH + H+ | - |
r | |
isopropanol + NAD+ | - |
Geobacillus thermodenitrificans | propan-2-one + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Geobacillus thermodenitrificans | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Geobacillus thermodenitrificans NG80-2 | formaldehyde + NADH + H+ | - |
r | |
octacosan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | octacosanal + NADH + H+ | - |
r | |
octadecan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | octadecanal + NADH + H+ | - |
r | |
octan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | octanal + NADH + H+ | - |
r | |
tetracosanol-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | tetracosanal + NADH + H+ | - |
r | |
tetradecan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | tetradecanal + NADH + H+ | - |
r | |
triacontan-1-ol + NAD+ | - |
Geobacillus thermodenitrificans | triacontanal + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 45000, SDS-PAGE, 8 * 41664, calculated from sequence | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
ADH1 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | - |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
14 h, more than 50% of initial activity | Geobacillus thermodenitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43.9 | - |
NADP+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
404.8 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
443.1 | - |
NAD+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
638.7 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans | |
1645.7 | - |
NADH | cosubstrate acetaldehyde, pH 8, 60°C | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ is about 2fold preferred over NADP+ | Geobacillus thermodenitrificans | |
NADH | - |
Geobacillus thermodenitrificans | |
NADP+ | NAD+ is about 2fold preferred over NADP+ | Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
84.6 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans | |
85.9 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
156.9 | - |
NADP+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
293.4 | - |
NAD+ | cosubstrate ethanol, pH 8, 60°C | Geobacillus thermodenitrificans | |
1175.5 | - |
NADH | cosubstrate acetaldehyde, pH 8, 60°C | Geobacillus thermodenitrificans |