Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
n-Propanol | 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C | Saccharolobus solfataricus | |
0.44 | - |
benzylalcohol | 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C | Saccharolobus solfataricus | |
1.33 | - |
ethanol | 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C | Saccharolobus solfataricus | |
2.4 | - |
iso-propanol | 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | ADH is a putative zinc-dependent alcohol dehydrogenase | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
deduced from amino acid sequence | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q9UXF1 | - |
- |
Saccharolobus solfataricus P2 | Q9UXF1 | - |
- |
Purification (Comment) | Organism |
---|---|
HIS-Select High Flow cartridge chromatography | Saccharolobus solfataricus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ethanol-grown cell | highest ethanol consumption rate in cultures grown on 0.79% w/v ethanol | Saccharolobus solfataricus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
crude extract, using benzylalcohol as substrate, at 65°C | Saccharolobus solfataricus |
0.18 | - |
after 5.67fold purification, using benzylalcohol as substrate, at 65°C | Saccharolobus solfataricus |
0.75 | - |
purified enzyme, using benzylalcohol as substrate, at 80°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylalcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH + H+ | - |
? | |
benzylalcohol + NAD+ | - |
Saccharolobus solfataricus P2 | benzaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus P2 | acetaldehyde + NADH + H+ | - |
? | |
iso-propanol + NAD+ | - |
Saccharolobus solfataricus | isopropanal + NADH + H+ | - |
? | |
iso-propanol + NAD+ | - |
Saccharolobus solfataricus P2 | isopropanal + NADH + H+ | - |
? | |
n-propanol + NAD+ | - |
Saccharolobus solfataricus | propanal + NADH + H+ | - |
? | |
n-propanol + NAD+ | - |
Saccharolobus solfataricus P2 | propanal + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH2 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
activity increases with temperature up to 95°C | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus |