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Information on EC 7.5.2.12 - ABC-type L-arabinose transporter
for references in articles please use BRENDA:EC7.5.2.12
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EC Tree 7 Translocases
7.5 Catalysing the translocation of carbohydrates and their derivatives
7.5.2 Linked to the hydrolysis of a nucleoside triphosphate
7.5.2.12 ABC-type L-arabinose transporter
IUBMB Comments
ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. A bacterial enzyme that interacts with an extracytoplasmic substrate binding protein and mediates the high-affinity uptake of L-arabinose.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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L-arabinose-[arabinose-binding protein][side 1]
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[arabinose-binding protein][side 1]
Synonyms
arafgh, arabinose transporter, high-affinity l-arabinose transport system, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
arabinose transporter
araFGH
ArAT
high-affinity L-arabinose symporter
L-arabinofuranose ABC transporter
L-arabinose ABC transporter
-
-
-
-
L-arabinose periplasmic permease
-
three components are associated with the L-arabinose periplasmic permease, the L-arabinose-binding protein (araF) and the two membrane-associated proteins encoded by araG and araH
L-arabinose transporter
L-arabinose transporting ATPase
-
-
-
-
L-arabinose-proton symporter
msmeg_1708-1712
Pc20g01790
Trire2_104072
araFGH
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1] = ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, L-arabinose-importing)
ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. A bacterial enzyme that interacts with an extracytoplasmic substrate binding protein and mediates the high-affinity uptake of L-arabinose.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + D-xylose-[xylose-binding protein][side 1]
ADP + phosphate + D-xylose[side 2] + [xylose-binding protein][side 1]
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
ATP + H2O + D-xylose-[xylose-binding protein][side 1]
ADP + phosphate + D-xylose[side 2] + [xylose-binding protein][side 1]
-
Substrates: the enzyme can also transport D-xylose but with low affinity (Km value about 9 mM)
Products: -
Products: -
?
ATP + H2O + D-xylose-[xylose-binding protein][side 1]
ADP + phosphate + D-xylose[side 2] + [xylose-binding protein][side 1]
-
Substrates: the enzyme can also transport D-xylose but with low affinity (Km value about 9 mM)
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
Substrates: high affinity and specificity for L-arabinose
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
Penicillium chrysogenum ATCC 28089
Substrates: high affinity and specificity for L-arabinose
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: the enzyme exhibits high affinity for L-arabinose
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: the enzyme exhibits high affinity for L-arabinose
Products: -
Products: -
?
additional information
?
-
-
Substrates: the ABC transporter has a wide range of substrates, including L-arabinose, D-fucose, D-galactose, D-glucose, and D-xylose
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme does not transport D-xylose or D-glucose
Products: -
Products: -
?
additional information
?
-
Penicillium chrysogenum ATCC 28089
Substrates: the enzyme does not transport D-xylose or D-glucose
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein MSMEG_1712][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein MSMEG_1712][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
Substrates: high affinity and specificity for L-arabinose
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
Penicillium chrysogenum ATCC 28089
Substrates: high affinity and specificity for L-arabinose
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1]
ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-xylose
-
in yeast, L-arabinose transport is inhibited slightly by D-xylose in an assay with 5fold excess of the inhibiting sugar
additional information
-
not inhibited by D-glucose in an assay with 5fold excess of the inhibiting sugar
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.13
L-arabinose-[arabinose-binding protein][side 1]
-
pH and temperature not specified in the publication
-
0.13
L-arabinose-[arabinose-binding protein][side 1]
pH and temperature not specified in the publication
-
0.207
L-arabinose-[arabinose-binding protein][side 1]
-
at pH 5.5 and 20°C
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the enzyme is not essential for growth of Trichoderma reesei on L-arabinose
physiological function
-
the enzyme is not essential for growth of Trichoderma reesei on L-arabinose
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ARAG1_BURCH
Burkholderia cenocepacia (strain HI2424)
503
0
55213
Swiss-Prot
-
ARAG2_BURCH
Burkholderia cenocepacia (strain HI2424)
515
0
55558
Swiss-Prot
-
ARAG_ECOL6
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
504
0
55018
Swiss-Prot
-
ARAG2_BURCM
Burkholderia ambifaria (strain ATCC BAA-244 / DSM 16087 / CCUG 44356 / LMG 19182 / AMMD)
519
0
55774
Swiss-Prot
-
ARAG1_BURCM
Burkholderia ambifaria (strain ATCC BAA-244 / DSM 16087 / CCUG 44356 / LMG 19182 / AMMD)
503
0
55117
Swiss-Prot
-
ARAG_ECOL5
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
504
0
55048
Swiss-Prot
-
ARAG_PARXL
Paraburkholderia xenovorans (strain LB400)
516
0
56634
Swiss-Prot
other Location (Reliability: 1)
ARAG2_BURO1
Burkholderia orbicola (strain AU 1054)
515
0
55558
Swiss-Prot
-
ARAG_YERPA
Yersinia pestis bv. Antiqua (strain Antiqua)
523
0
56873
Swiss-Prot
-
ARAG_YERPN
Yersinia pestis bv. Antiqua (strain Nepal516)
523
0
56873
Swiss-Prot
-
ARAG1_BURO1
Burkholderia orbicola (strain AU 1054)
503
0
55213
Swiss-Prot
-
ARAG_CHRSD
Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11)
497
0
55078
Swiss-Prot
-
ARAG_RHIJ3
Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841)
502
0
54429
Swiss-Prot
-
ARAG_ECOUT
Escherichia coli (strain UTI89 / UPEC)
504
0
55018
Swiss-Prot
-
ARAG_RHIEC
Rhizobium etli (strain ATCC 51251 / DSM 11541 / JCM 21823 / NBRC 15573 / CFN 42)
501
0
54502
Swiss-Prot
-
ARAG2_BURTA
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)
525
0
56397
Swiss-Prot
other Location (Reliability: 2)
ARAG1_BURTA
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)
503
0
55040
Swiss-Prot
-
ARAG_SHIDS
Shigella dysenteriae serotype 1 (strain Sd197)
504
0
55046
Swiss-Prot
-
ARAG_SHIBS
Shigella boydii serotype 4 (strain Sb227)
504
0
54991
Swiss-Prot
-
ARAG1_BURL3
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
503
0
55178
Swiss-Prot
-
ARAG2_BURL3
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
522
0
56222
Swiss-Prot
-
ARAG_BURP1
Burkholderia pseudomallei (strain 1710b)
503
0
55034
Swiss-Prot
Secretory Pathway (Reliability: 3)
ARAG_PSEPF
Pseudomonas fluorescens (strain Pf0-1)
514
0
55502
Swiss-Prot
-
ARAG_PSE14
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
507
0
55584
Swiss-Prot
-
ARAG_PSEU2
Pseudomonas syringae pv. syringae (strain B728a)
507
0
55495
Swiss-Prot
other Location (Reliability: 1)
ARAG_BURPS
Burkholderia pseudomallei (strain K96243)
503
0
54965
Swiss-Prot
-
ARAG_BURMA
Burkholderia mallei (strain ATCC 23344)
503
0
55081
Swiss-Prot
-
ARAG_MANSM
Mannheimia succiniciproducens (strain KCTC 0769BP / MBEL55E)
502
0
55539
Swiss-Prot
-
ARAG_YERPS
Yersinia pseudotuberculosis serotype I (strain IP32953)
523
0
56873
Swiss-Prot
-
ARAG_PECAS
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
507
0
55508
Swiss-Prot
-
ARAG_VIBPA
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
506
0
55733
Swiss-Prot
other Location (Reliability: 3)
ARAG_PSESM
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
507
0
55301
Swiss-Prot
-
ARAG_RALN1
Ralstonia nicotianae (strain ATCC BAA-1114 / GMI1000)
511
0
55936
Swiss-Prot
-
ARAG_HALH5
Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
517
0
57148
Swiss-Prot
-
ARAG_GEOSE
513
0
57160
Swiss-Prot
other Location (Reliability: 2)
B6HE12_PENRW
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)
511
0
56260
TrEMBL
-
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 33200 + x * 54947 + x * 34309, subunits araF, araG and araH, calculated from amino acid sequence
additional information
-
the high-affinity L-arabinose transport operon: araF encodes a 35512 Da L-arabinose-binding protein, the araG gene encodes a 54947 Da protein and the araH gene encodes a 34309 Da protein
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
-
the L-arabinose binding protein encoded by araF contains a signal peptide that is cleaved upon formation of the mature protein
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strains IMX658 and DS68625
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is highly expressed on L-arabinose
the expression of GguA is up-regulated in the presence of L-arabinose, D-fucose, and D-galactose
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, M.; Mueller, C.; Froehlich, K.; Gorka, O.; Zhang, L.; Gross, O.; Schilling, O.; Einsle, O.; Jessen-Trefzer, C.
Detection and characterization of a mycobacterial L-arabinofuranose ABC transporter identified with a rapid lipoproteomics protocol
Cell Chem. Biol.
26
852-862.e6
2019
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2155
brenda
Horazdovsky, B.F.; Hogg, R.W.
Genetic reconstitution of the high-affinity L-arabinose transport system
J. Bacteriol.
171
3053-3059
1989
Escherichia coli
brenda
Zhao, J.; Binns, A.N.
Characterization of the mmsAB-araD1 (gguABC) genes of Agrobacterium tumefaciens
J. Bacteriol.
193
6586-6596
2011
Agrobacterium tumefaciens
brenda
Scripture, J.B.; Voelker, C.; Miller, S.; O'Donnell, R.T.; Polgar, L.; rade, J.; Horazdovsky, B.F.; Hogg, R.W.
High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products
J. Mol. Biol.
197
37-46
1987
Escherichia coli
brenda
Bracher, J.M.; Verhoeven, M.D.; Wisselink, H.W.; Crimi, B.; Nijland, J.G.; Driessen, A.J.M.; Klaassen, P.; van Maris, A.J.A.; Daran, J.G.; Pronk, J.T.
The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive L-arabinose transport in Saccharomyces cerevisiae
Biotechnol. Biofuels
11
63
2018
Penicillium chrysogenum (B6HE12), Penicillium chrysogenum ATCC 28089 (B6HE12)
brenda
Verhoeven, M.; Bracher, J.; Nijland, J.; Bouwknegt, J.; Daran, J.; Driessen, A.; Van Maris, A.; Pronk, J.
Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive L-arabinose uptake
FEMS Yeast Res.
18
foy062
2018
Penicillium chrysogenum
brenda
Havukainen, S.; Pujol-Gimenez, J.; Valkonen, M.; Hediger, M.A.; Landowski, C.P.
Functional characterization of a highly specific L-arabinose transporter from Trichoderma reesei
Microb. Cell Fact.
20
177
2021
Trichoderma reesei, Trichoderma reesei QM6a
brenda
EXTERNAL LINKS (specific for EC-Number 7.5.2.12)
Transporter Classification Database (TCDB): 3.A.1.2.2
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