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Information on EC 6.5.1.B3 - 2'-5' RNA ligase (GTP-activated) and Organism(s) Pyrococcus horikoshii and UniProt Accession O57823

for references in articles please use BRENDA:EC6.5.1.B3
preliminary BRENDA-supplied EC number
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Pyrococcus horikoshii
UNIPROT: O57823
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The taxonomic range for the selected organisms is: Pyrococcus horikoshii
The expected taxonomic range for this enzyme is: Pyrococcus
Reaction Schemes
a 3'-half-tRNA molecule with a 5'-oH end
+
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
=
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
Synonyms
pf0027, gtp-dependent rna ligase, ph0099, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 3'-half-tRNA molecule with a 5'-oH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end = a mature tRNA molecule containing a 2'-5'-phosphodiester bond
show the reaction diagram
ligation reaction is dependent on GTP
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure solved at 1.94 A resolution, structural comparison wuth the 2',5' RNA ligase from Thermus thermophilus
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gao, Y.G.; Yao, M.; Okada, A.; Tanaka, I.
The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94 A resolution reveals a possible open form with a wider active-site cleft
Acta Crystallogr. Sect. F
62
1196-1200
2006
Pyrococcus horikoshii (O57823), Pyrococcus horikoshii, Pyrococcus horikoshii OT-3 (O57823)
Manually annotated by BRENDA team