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Information on EC 5.3.1.17 - 5-dehydro-4-deoxy-D-glucuronate isomerase and Organism(s) Escherichia coli and UniProt Accession Q46938

for references in articles please use BRENDA:EC5.3.1.17
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IUBMB Comments
The enzyme is involved in the degradation of polygalacturonate, a later stage in the degradation of pectin by many microorganisms.
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This record set is specific for:
Escherichia coli
UNIPROT: Q46938
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
5-keto-4-deoxyuronate isomerase, 4-deoxy-l-threo-5-hexosulose-uronate ketol-isomerase, 5-keto 4-deoxyuronate isomerase, gbs1892, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-keto-4-deoxyuronate isomerase
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4-Deoxy-L-threo-5-hexosulose uronic acid isomerase
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-
-
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4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
5-keto 4-deoxyuronate isomerase
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-
5-keto-4-deoxyuronate isomerase
DKI isomerase
-
-
-
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Isomerase, 4-deoxy-L-threo-5-hexulose uronate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
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intramolecular oxidoreduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5-dehydro-4-deoxy-D-glucuronate aldose-ketose-isomerase
The enzyme is involved in the degradation of polygalacturonate, a later stage in the degradation of pectin by many microorganisms.
CAS REGISTRY NUMBER
COMMENTARY hide
37318-44-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-dehydro-4-deoxy-D-glucuronate
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
show the reaction diagram
-
-
-
?
5-dehydro-4-deoxy-D-glucuronate
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-dehydro-4-deoxy-D-glucuronate
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
show the reaction diagram
-
-
-
?
5-dehydro-4-deoxy-D-glucuronate
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
the KduI enzyme facilitates the conversion of galacturonate and glucuronate and facilitates the growth of Escherichia coli on galacturonate and glucuronate in the presence of osmotically active sucrose
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
165000
hexamer, dynamic light scattering measurements
188000
gel filtration and analytical ultracentrifugation
290000
trace amounts, gel filtration and analytical ultracentrifugation
31000
6 * 31000, crystal structure, SDS-PAGE
75000
trace amounts, gel filtration and analytical ultracentrifugation
31000
-
x * 31000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 31000, crystal structure, SDS-PAGE
?
-
x * 31000, SDS-PAGE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-CAM chelating resin chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli, strain B348
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli JM109 or KRX cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
50 mM glucuronate induces kduI gene expression 7-11fold under aerobic conditions and 19-54fold under anaerobic conditions
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Crowther, R.L.; Georgiadis, M.M.
The crystal structure of 5-keto-4-deoxyuronate isomerase from Escherichia coli
Proteins
61
680-684
2005
Escherichia coli (Q46938), Escherichia coli
Manually annotated by BRENDA team
Rothe, M.; Alpert, C.; Loh, G.; Blaut, M.
Novel insights into E. colis hexuronate metabolism: KduI facilitates the conversion of galacturonate and glucuronate under osmotic stress conditions
PLoS ONE
8
e56906
2013
Escherichia coli
Manually annotated by BRENDA team
Rodina, E.; Vorobieva, N.; Kurilova, S.; Mikulovich, J.; Vainonen, J.; Aro, E.M.; Nazarova, T.
Identification of new protein complexes of Escherichia coli inorganic pyrophosphatase using pull-down assay
Biochimie
93
1576-1583
2011
Escherichia coli
Manually annotated by BRENDA team
Dadinova, L.A.; Shtykova, E.V.; Konarev, P.V.; Rodina, E.V.; Snalina, N.E.; Vorobyeva, N.N.; Kurilova, S.A.; Nazarova, T.I.; Jeffries, C.M.; Svergun, D.I.
X-Ray solution scattering study of four Escherichia coli enzymes involved in stationary-phase metabolism
PLoS ONE
11
e0156105
2016
Escherichia coli (Q46938), Escherichia coli
Manually annotated by BRENDA team