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IUBMB CommentsThe enzyme, characterized from the bacterium Actinomyces sp. Lu 9419, is involved in the biosynthesis of the antitumor agent cetoniacytone A.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
2-epi-5-epi-valiolone epimerase,
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CetB
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EVE
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2-epi-5-epi-valiolone = 5-epi-valiolone
2-epi-5-epi-valiolone = 5-epi-valiolone
proposed reaction mechanism
2-epi-5-epi-valiolone = 5-epi-valiolone
proposed reaction mechanism
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2-epi-5-epi-valiolone = 5-epi-valiolone
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2-epi-5-epi-valiolone 2-epimerase
The enzyme, characterized from the bacterium Actinomyces sp. Lu 9419, is involved in the biosynthesis of the antitumor agent cetoniacytone A.
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2-epi-5-epi-valiolone
5-epi-valiolone
2-epi-5-epi-valiolone
5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
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-
?
2-epi-5-epi-valiolone
5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
-
-
?
2-epi-5-epi-valiolone
5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
-
-
?
2-epi-5-epi-valiolone
5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
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-
?
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2-epi-5-epi-valiolone
5-epi-valiolone
2-epi-5-epi-valiolone
5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
-
-
?
2-epi-5-epi-valiolone
5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
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-
?
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Co2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
Ni2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
additional information
each monomer contains two beta,alpha,beta,beta,beta scaffolds that form a metal binding site with two histidine and two glutamic acid residues
additional information
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each monomer contains two beta,alpha,beta,beta,beta scaffolds that form a metal binding site with two histidine and two glutamic acid residues
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UniProt
brenda
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UniProt
brenda
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physiological function
the enzyme is involved in the biosynthesis of cetoniacytone A
physiological function
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the enzyme is involved in the biosynthesis of cetoniacytone A
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CETB_ACTSX
Actinomyces sp
183
0
20047
Swiss-Prot
-
A0A2H5VZI9_9BACT
137
0
15021
TrEMBL
-
A0A2H6B2Z5_9BACT
134
0
14448
TrEMBL
-
A0A0A6FVM6_ENTCL
129
0
14535
TrEMBL
-
A0A2I2KIE0_9ACTN
155
0
16807
TrEMBL
-
A0A385XUC5_9GAMM
129
0
14561
TrEMBL
-
A0A7U9WTY4_9FIRM
130
0
14685
TrEMBL
-
A0A509EGI8_9HYPH
176
0
19271
TrEMBL
-
A0A5S9R3Y8_MYCVN
174
0
19640
TrEMBL
-
A0A7U9XPH5_9FIRM
130
0
14671
TrEMBL
-
A0A399EBS6_9DEIN
142
0
15877
TrEMBL
-
A0A446C5D8_9BURK
150
0
17374
TrEMBL
-
A0A172XP56_9BACI
127
0
14455
TrEMBL
-
A0A2S6R181_9PROT
191
0
21455
TrEMBL
-
A0A7U2ZPS9_9ENTR
129
0
14653
TrEMBL
-
A0A220W449_9SPHN
144
0
15581
TrEMBL
-
A0A8J4X5N2_9BACT
165
0
17808
TrEMBL
-
A0A2S6R0T9_9PROT
150
0
16268
TrEMBL
-
A0A220W5V9_9SPHN
141
0
15391
TrEMBL
-
A0A7G3EZE3_ENTCL
173
0
19259
TrEMBL
-
A0A117P4R0_9ACTN
146
0
16231
TrEMBL
-
A0A418SER8_9RHOB
129
0
14579
TrEMBL
-
A0A3S1A9P8_9GAMM
129
0
14618
TrEMBL
-
A0A5S9PIM3_9GAMM
166
0
19206
TrEMBL
-
A0A5J4E3T0_9BACT
142
0
15224
TrEMBL
-
A0A8H9CKI5_9PSED
182
0
20614
TrEMBL
-
A0A2P5VK22_9PROT
150
0
16179
TrEMBL
-
A0A2S6R8R4_9PROT
190
0
21629
TrEMBL
-
A0A220WAH3_9SPHN
165
0
18586
TrEMBL
-
A0A6S7D2Y5_9BURK
165
0
18139
TrEMBL
-
A0A653JL10_9SPHN
165
0
18459
TrEMBL
-
A0A6S7F4A8_9BURK
150
0
17088
TrEMBL
-
A0A6P1PTV4_9GAMM
129
0
14713
TrEMBL
-
A0A5E7II78_PSEFL
180
0
19598
TrEMBL
-
A0A653JFP5_9SPHN
144
0
15650
TrEMBL
-
A0A6S7AL05_9BURK
195
1
20845
TrEMBL
-
A0A2H5UZK2_9ARCH
158
0
18228
TrEMBL
-
A0A5E7VGH8_PSEFL
180
0
19517
TrEMBL
-
A0A2W1JPM3_9CYAN
Acaryochloris thomasi RCC1774
302
0
33644
TrEMBL
-
A0A6P2G683_9BURK
181
0
20106
TrEMBL
-
A0A387HHV0_9ACTN
146
0
16066
TrEMBL
-
A0A2H5VJE8_9BACT
138
0
15195
TrEMBL
-
A0A6V7R0K0_9STAP
177
0
20154
TrEMBL
-
A0A446C8J4_9BURK
150
0
17345
TrEMBL
-
A0A2S6RWB0_9PROT
151
0
16277
TrEMBL
-
A0A644TL11_9ZZZZ
145
0
15949
TrEMBL
other Location (Reliability: 2)
A0A7D7KZD8_KOCVA
307
0
33038
TrEMBL
-
A0A2Z2NTP0_9GAMM
166
0
19190
TrEMBL
-
A0A857JK47_9ALTE
195
0
22141
TrEMBL
-
A0A2S6XFT3_9ACTN
167
0
18995
TrEMBL
-
A0A2S6XCC5_9ACTN
146
0
16010
TrEMBL
-
A0A2H5YN17_9BACT
172
0
19121
TrEMBL
-
A0A6S7D533_9BURK
150
0
17147
TrEMBL
-
A0A5P9Q9M5_9MICO
144
0
15809
TrEMBL
-
A0A6N7CJ67_9NOCA
183
0
20437
TrEMBL
-
A0A399E7U6_9DEIN
142
0
16001
TrEMBL
-
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24000
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
48000
polyhistidine-tagged protein, gel filtration, nondenaturing-PAGE
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homodimer
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
homodimer
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2 * 24000, polyhistidine-tagged protein, SDS-PAGE
-
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E151G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
E76G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
E76G/E151G
inactive mutant enzyme
E76G/H99G
inactive mutant enzyme
H14G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
H14G/E151G
inactive mutant enzyme
H14G/E76G
inactive mutant enzyme
H14G/H99G
inactive mutant enzyme
H99G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
H99G/E151G
inactive mutant enzyme
E151G
-
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
-
E76G
-
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
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H14G
-
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
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H14G/E76G
-
inactive mutant enzyme
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H99G
-
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
-
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overexpression in Escherichia coli BL21(DE3)pLysS
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Wu, X.; Flatt, P.M.; Xu, H.; Mahmud, T.
Biosynthetic gene cluster of cetoniacytone A, an unusual aminocyclitol from the endosymbiotic bacterium Actinomyces sp. Lu 9419
Chembiochem
10
304-314
2009
Actinomyces sp. (A1YPR3), Actinomyces sp., Actinomyces sp. Lu 9419 (A1YPR3)
brenda
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5.1.3.33 2-epi-5-epi-valiolone epimerase
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