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Information on EC 4.2.3.99 - labdatriene synthase and Organism(s) Oryza sativa and UniProt Accession Q2QQJ5

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.99 labdatriene synthase
IUBMB Comments
The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
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This record set is specific for:
Oryza sativa
UNIPROT: Q2QQJ5
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The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Oryza sativa
Synonyms
KSL10, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase [(12E)-9alpha-labda-8(17),12,14-triene-forming]
The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
syn-copalyl diphosphate
syn-labda-8(17),12E,14-triene + diphosphate
show the reaction diagram
-
i.e. (4aS,5R,8aS)-1,1,4a-trimethyl-6-methylidene-5-[(2E)-3-methylpenta-2,4-dien-1-yl]decahydronaphthalene
-
?
additional information
?
-
additionally converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene, reaction of EC 4.2.3.29
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
KM value for substrate ent-copalyl diphosphate is 0.0006 mM
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00083
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
Kcat value for substrate ent-copalyl diphosphate is 0.00033 per s
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
Kcat/KM value for substrate ent-copalyl diphosphate is 0.55 per s and mM
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression as glutathione-S-transferase fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morrone, D.; Hillwig, M.L.; Mead, M.E.; Lowry, L.; Fulton, D.B.; Peters, R.J.
Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals
Biochem. J.
435
589-595
2011
Oryza sativa (Q2QQJ5)
Manually annotated by BRENDA team