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Information on EC 4.2.3.85 - alpha-eudesmol synthase and Organism(s) Zingiber zerumbet and UniProt Accession B1B1U4

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.85 alpha-eudesmol synthase
IUBMB Comments
The recombinant enzyme from ginger (Zingiber zerumbet) gives 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene. cf. EC 4.2.3.68 (beta-eudesmol synthase) and EC 4.2.3.84 (10-epi-gamma-eudesmol synthase)
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Zingiber zerumbet
UNIPROT: B1B1U4
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The taxonomic range for the selected organisms is: Zingiber zerumbet
The expected taxonomic range for this enzyme is: Zingiber zerumbet
Synonyms
ZSS2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (alpha-eudesmol-forming)
The recombinant enzyme from ginger (Zingiber zerumbet) gives 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene. cf. EC 4.2.3.68 (beta-eudesmol synthase) and EC 4.2.3.84 (10-epi-gamma-eudesmol synthase)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
alpha-eudesmol + diphosphate
show the reaction diagram
-
products are 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. Smith
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong expression, transcript levels increase rapidly in summer, peak in early August and decline significantly in fall and winter
Manually annotated by BRENDA team
poor expression
Manually annotated by BRENDA team
additional information
no expression in leaf
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSBE_ZINZE
554
0
64382
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yu, F.; Harada, H.; Yamasaki, K.; Okamoto, S.; Hirase, S.; Tanaka, Y.; Misawa, N.; Utsumi, R.
Isolation and functional characterization of a beta-eudesmol synthase, a new sesquiterpene synthase from Zingiber zerumbet Smith
FEBS Lett.
582
565-572
2008
Zingiber zerumbet (B1B1U4)
Manually annotated by BRENDA team