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Information on EC 4.2.3.75 - (-)-germacrene D synthase and Organism(s) Vitis vinifera and UniProt Accession Q6Q3H3

for references in articles please use BRENDA:EC4.2.3.75
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.75 (-)-germacrene D synthase
IUBMB Comments
In Solidago canadensis the biosynthesis results in the pro-R hydrogen at C-1 of the farnesy diphosphate ending up at C-11 of the (-)-germacrene D . With Streptomyces coelicolor the pro-S hydrogen at C-1 ends up at C-11 of the (-)-germacrene D .
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This record set is specific for:
Vitis vinifera
UNIPROT: Q6Q3H3
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Word Map
The taxonomic range for the selected organisms is: Vitis vinifera
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
terpenoid cyclase, (-)-germacrene d synthase, tps07, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(-)-germacrene D synthase
-
germacrene D synthase
-
terpenoid cyclase
-
ylangene/germacrene synthase
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(-)-germacrene-D-forming]
In Solidago canadensis the biosynthesis results in the pro-R hydrogen at C-1 of the farnesy diphosphate ending up at C-11 of the (-)-germacrene D [1]. With Streptomyces coelicolor the pro-S hydrogen at C-1 ends up at C-11 of the (-)-germacrene D [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-germacrene D + alpha-ylangene + diphosphate
show the reaction diagram
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-germacrene D + diphosphate
show the reaction diagram
-
plus about 8% dleta-cadinene
-
?
2-trans,6-trans-farnesyl diphosphate
(-)-(7S)-germacrene D + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-germacrene D + alpha-ylangene + diphosphate
show the reaction diagram
-
-
-
?
2-trans,6-trans-farnesyl diphosphate
(-)-(7S)-germacrene D + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
vegetative tissues of young leaves
Manually annotated by BRENDA team
green stem
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSGD_VITVI
557
0
64269
Swiss-Prot
Chloroplast (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64300
x * 64300, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64300, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis of Vitis vinifera cv. Gewürztraminer, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)-RIL
expressed in Nicotiana benthamiana
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luecker, J.; Bowen, P.; Bohlmann, J.
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Phytochemistry
65
2649-2659
2004
Vitis vinifera (Q6Q3H3), Vitis vinifera
Manually annotated by BRENDA team
Dueholm, B.; Drew, D.P.; Sweetman, C.; Simonsen, H.T.
In planta and in silico characterization of five sesquiterpene synthases from Vitis vinifera (cv. Shiraz) berries
Planta
249
59-70
2019
Vitis vinifera (Q6Q3H3), Vitis vinifera
Manually annotated by BRENDA team